Liquid nitrogen (~-200 ºC) |
Glycerol |
Glycerol (50%, w/v) |
scFv antibody |
Cunha et al.2525 Cunha AE, Clemente JJ, Gomes R, et al. Methanol induction optimization for scFv antibody fragment production in Pichia pastoris. Biotechnol Bioeng. 2004;86:458-467.
|
-80 ºC |
Glycerol |
Glycerol |
6-Fructosyl transferase |
Hochstrasser et al.2828 Hochstrasser U, Lüscher M, De Virgilio C, Boller T, Wiemken A. Expression of a functional barley sucrose-fructan 6-fructosyltransferase in the methylotrophic yeast Pichia pastoris. FEBS Lett. 1998;440:356-360.
|
N/I |
N/I |
Glycerol |
Glycerol 3-phosphate dehydrogenase |
Peres et al.3434 Peres MFS, Silva VC, Valentini SR, Gattás EAL. Recombinant expression of glycerol-3-phosphate dehydrogenase using the Pichia pastoris system. J Mol Catal B Enzym. 2010;65:128-132.
|
N/I |
N/I |
Glycerol (1%) |
Anti CTLA4 single chain variable fragment |
Cai et al.2424 Cai H, Chen L, Wan L, et al. High-level expression of a functional humanized anti-CTLA4 single-chain variable fragment antibody in Pichia pastoris. Appl Microbiol Biotechnol. 2009;82:41-48.
|
-80 ºC |
Glycerol (24%) |
Glycerol |
Trypsinogen |
Paulová et al.3333 Paulová L, Hyka P, Branská B, Melzoch K, Kovar K. Use of a mixture of glucose and methanol as substrates for the production of recombinant trypsinogen in continuous cultures with Pichia pastoris Mut+. J Biotechnol. 2012;157:180-188.
|
-70 ºC |
Glycerol 20% |
Glycerol |
scFv antibody |
Zepeda et al.1818 Zepeda AB, Figueroa CA, Abdalla DSP, et al. Biomarkers to evaluate the effects of temperature and methanol on recombinant Pichia pastoris. Braz J Microbiol. 2014;45:475-483.
|
-70 ºC |
Glycerol |
Glucose/glycerol (20 g/L) |
Iduronate 2-sulfate sulfatase |
Poutou et al.3636 Poutou PRA, Quevedo HBE, Córdoba HA, Sáenz H, Barrera ALA. Influencia de la fuente de carbono sobre la expresión de proteínas AOX1-reguladas en Pichia pastoris. Nova. 2005;3(3):75-87.
|
Frozen culture |
Glycerol |
Glycerol |
Ovine interferon |
Sinha et al.3737 Sinha J, Plantz BA, Inan M, Meagher MM. Causes of proteolytic degradation of secreted recombinant proteins produced in methylotrophic yeast Pichia pastoris: case study with recombinant ovine interferon-τ. Biotechnol Bioeng. 2005;89:102-112.
|
N/I |
N/I |
Glycerol |
Cancer-testis antigen SSX2 |
Huang et al.2929 Huang C-J, Anderson KA, Damasceno LM, Ritter G, Old LJ, Batt CA. Improved secretion of the cancer-testis antigen SSX2 in Pichia pastoris by deletion of its nuclear localization signal. Appl Microbiol Biotechnol. 2010;86:243-253.
|
N/I |
N/I |
Glucose |
scFv antibody |
Miller et al.3232 Miller KD, Weaver-Feldhaus J, Gray SA, Siegel RW, Feldhaus MJ. Production, puriWcation, and characterization of human scFv antibodies expressed in Saccharomyces cerevisiae, Pichia pastoris, and Escherichia coli. Protein Expr Purif. 2005;42:255-267.
|
-80 ºC |
Glycerol |
Glycerol (1%, v/v) |
scFv antibody |
Damasceno et al.2626 Damasceno LM, Pla I, Chang HJ, et al. An optimized fermentation process for high-level production of a single-chain Fv antibody fragment in Pichia pastoris. Protein Expr Purif. 2004;37:18-26.
|
Frozen cell stock |
N/I |
Dextrose |
scFv antibody |
Yamawaki et al.3939 Yamawaki S, Matsumoto T, Ohnishi Y, et al. Production of single-chain variable fragment antibody (scFv) in fed-batch and continuous culture of Pichia pastoris by two different methanol feeding methods. J Biosci Bioeng. 2007;104:403-407.
|
-80 ºC |
Glycerol |
Glycerol |
Single chain antibody |
Khatri and Hoffmann3030 Khatri NK, Hoffmann F. Impact of methanol concentration on secreted protein production in oxygen-limited cultures of recombinant Pichia pastoris. Biotechnol Bioeng. 2006;93:871-879.
|
N/I |
N/I |
Glycerol |
scFv antibody |
Khatri and Hoffmann3131 Khatri NK, Hoffmann F. Oxygen-limited control of methanol uptake for improved production of a single-chain antibody fragment with recombinant Pichia pastoris. Appl Microbiol Biotechnol. 2006;72:492-498.
|
N/I |
N/I |
Glycerol |
Glycerol kinase |
Aizemberg et al.2323 Aizemberg R, Terrazas WDM, Ferreira-Dias S, Valentini SR, Gattás EAL. Recombinant glycerol kinase production by P. pastoris. Food Technol Biotechnol. 2011;49:329-335.
|
Liquid nitrogen (~-200 ºC) |
Glycerol (15%) |
Glycerol |
Heavy chain C-terminal fragment of botulinum neurotoxin serotype E |
Sinha et al.3838 Sinha J, Inan M, Fanders S. Cell bank characterization and fermentation optimization for production of recombinant heavy chain C-terminal fragment of botulinum neurotoxin serotype E (rBoNTE(H(c)): antigen E) by Pichia pastoris. J Biotechnol. 2007;127:462-474.
|
Frozen cell stock |
N/I |
Glycerol (40 g/L) |
hg-CSF |
Bahrami et al.1717 Bahrami A, Shojaosadati SA, Khalilzadeh R, Farahani EV. Two-stage glycerol feeding for enhancement of recombinant hG-CSF production in a fed-batch culture of Pichia pastoris. Biotechnol Lett. 2008;30:1081-1085.
|
N/I |
N/I |
Glycerol |
Sea raven antifreeze |
d'Anjou and Daugulis2727 d'Anjou MC, Daugulis AJ. A rational approach to improving productivity in recombinant Pichia pastoris fermentation. Biotechnol Bioeng. 2001;72:1-11.
|
N/I |
N/I |
Glycerol |
Green fluorescent protein |
Yang et al.4040 Yang J, Nie L, Chen B, et al. Hygromycin-resistance vectors for gene expression in Pichia pastoris. Yeast. 2014;31:115-125.
|
N/I |
N/I |
Glycerol |
rbLf To |
Iglesias-Figueroa et al.1212 Iglesias-Figueroa B, Valdiviezo-Godina N, Siqueiros-Cendón T, Sinagawa-García S, Arévalo-Gallegos S, Rascón-Cruz Q. High-level expression of recombinant bovine lactoferrin in Pichia pastoris with antimicrobial activity. Int J Mol Sci. 2016;17:E902.
|
N/I |
N/I |
Glycerol |
INVAAOX1 and INVBAOX
|
Pérez de los Santos et al.3535 Pérez de los Santos AI, Cayetano-Cruz M, Gutiérrez-Antón M, et al. Improvement of catalytical properties of two invertases highly tolerant to sucrose after expression in Pichia pastoris. Effect of glycosylation on enzyme properties. Enzyme Microb Technol. 2016;83:48-56.
|