Endo-1,4-β-mannanase |
GS115 |
AOX1 |
Methanol |
28 ºC |
0.262 mg ml-1
|
5353 Zhong Y, Yang L, Guo Y, et al. High-temperature cultivation of recombinant Pichia pastoris increases endoplasmic reticulum stress and decreases production of human interleukin-10. Microb Cell Fact. 2014;13:163; Liao H, Li S, Zheng H, et al. A new acidophilic thermostableendo thermostable endo-1,4-β-mannanase from Penicillium oxalicum GZ-2: cloning, characterization and functional expression in Pichia pastoris. BMC Biotechnol. 2014;14:90.
|
Bovine β-lactoglobulin |
GS115 |
AOX1 |
Methanol |
30 ºC |
1 mg ml-1
|
5454 Kim TR, Goto Y, Hirota N, et al. High-level expression of bovine beta-lactoglobulin in Pichia pastoris and characterization of its physical properties. Protein Eng. 1997;10(11):1339-1345.
|
Human collagen α1(III) chain |
GS115 |
AOX1 |
Methanol |
28.5 ºC |
3.36 mg ml-1
|
5555 Li L, Fan D, Ma X, Deng J, He J. High-level secretory expression and purification of unhydroxylated human collagen α1(III) chain in Pichia pastoris GS115. Biotechnol Appl Biochem. 2015;62(4):467-475.
|
Porcine lactoferrin |
SMD 1168 |
GAP |
Sorbitol |
30 ºC |
0.012 mg ml-1
|
5656 Wang SH, Yang TS, Lin SM, Tsai MS, Wu SC, Mao SJ. Expression, characterization, and purification of recombinant porcine lactoferrin in Pichia pastoris. Protein Expr Purif. 2002;25(1):41-49.
|
Vitreoscilla hemoglobin |
X-33 |
ADH2 |
Glucose |
30 ºC |
16 nmol/g dry cell |
5757 Chien LJ, Lee CK. Expression of bacterial hemoglobin in the yeast, Pichia pastoris, with a low O2-induced promoter. Biotechnol Lett. 2005;27(19):1491-1497.
|
Phytase C (PhyC-R) |
GS115 |
AOX1 |
Methanol |
30 ºC |
0.71 mg ml-1
|
5858 Guerrero-Olazarán M, Rodríguez-Blanco L, Carreon-Treviño JG, Gallegos-López JA, Viader-Salvadó JM. Expression of a Bacillus phytase C gene in Pichia pastoris and properties of the recombinant enzyme. Appl Environ Microbiol. 2010;76(16):5601-5608.
|
Lipase |
X-33 |
FLD1 |
Glycerol |
30 ºC |
2.5 mg ml-1
|
2323 Resina D, Serrano A, Valero F, Ferrer P. Expression of a Rhizopus oryzae lipase in Pichia pastoris under control of the nitrogen source-regulated formaldehyde dehydrogenase promoter. J Biotechnol. 2004;109(1–2):103-113.
|
Lipase |
X-33 |
FLD1 |
Sorbitol |
30 ºC |
3.3 mg ml-1
|
2323 Resina D, Serrano A, Valero F, Ferrer P. Expression of a Rhizopus oryzae lipase in Pichia pastoris under control of the nitrogen source-regulated formaldehyde dehydrogenase promoter. J Biotechnol. 2004;109(1–2):103-113.
|
Lipase |
X-33 |
FLD1 |
Methanol |
30 ºC |
2.4 mg ml-1
|
2323 Resina D, Serrano A, Valero F, Ferrer P. Expression of a Rhizopus oryzae lipase in Pichia pastoris under control of the nitrogen source-regulated formaldehyde dehydrogenase promoter. J Biotechnol. 2004;109(1–2):103-113.
|
Lipase LIP2 (YlLIP2) |
X-33 |
GAP |
Glucose |
28 ºC |
120 g DCW/l |
4343 Wang X, Sun Y, Ke F, et al. Constitutive expression of Yarrowia lipolytica lipase LIP2 in Pichia pastoris using GAP as promoter. Appl Biochem Biotechnol. 2012;166(5):1355-1367.
|
Lipase |
X-33 |
AOX1 |
Methanol |
30 ºC |
60.7 mg ml-1
|
5959 Cos O, Serrano A, Montesinos JL, Ferrer P, Cregg JM, Valero F. Combined effect of the methanol utilization (Mut) phenotype and gene dosage on recombinant protein production in Pichia pastoris fed-batch cultures. J Biotechnol. 2005;116(4):321-335.
|
Lipase |
KM71 |
AOX2 |
Methanol |
30 ºC |
35.5 mg ml-1
|
5959 Cos O, Serrano A, Montesinos JL, Ferrer P, Cregg JM, Valero F. Combined effect of the methanol utilization (Mut) phenotype and gene dosage on recombinant protein production in Pichia pastoris fed-batch cultures. J Biotechnol. 2005;116(4):321-335.
|
Lipase |
X-33 |
FLD1 |
Sorbitol |
30 ºC |
50.4 mg ml-1
|
2626 Resina D, Cos O, Ferrer P, Valero F. Developing high cell density fed-batch cultivation strategies for heterologous protein production in Pichia pastoris using the nitrogen source-regulated FLD1 promoter. Biotechnol Bioeng. 2005;91(6):760-767.
|
rhIL-2-HSA fusion protein |
GS115 |
GAP |
Glucose |
25 ºC |
0.25 mg ml-1
|
6060 Guan B, Chen F, Lei J, et al. Constitutive expression of a rhIL-2-HSA fusion protein in Pichia pastoris using glucose as carbon source. Appl Biochem Biotechnol. 2013;171(7):1792-1804.
|
AfCel12A endonuclease |
PichiaPink™ |
GAP |
Glycerol |
25 ºC |
7.3 mg ml-1
|
6161 Várnai A, Tang C, Bengtsson O, Atterton A, Mathiesen G, Eijsink VG. Expression of endoglucanases in Pichia pastoris under control of the GAP promoter. Microb Cell Fact. 2014;13(1):57.
|
TαCel5A endonuclease |
PichiaPink™ |
GAP |
Glycerol |
25 ºC |
5.4 mg ml-1
|
6161 Várnai A, Tang C, Bengtsson O, Atterton A, Mathiesen G, Eijsink VG. Expression of endoglucanases in Pichia pastoris under control of the GAP promoter. Microb Cell Fact. 2014;13(1):57.
|
TαCel5A endonuclease |
PichiaPink™ |
GAP |
Glucose |
30 ºC |
4.9 mg ml-1
|
6161 Várnai A, Tang C, Bengtsson O, Atterton A, Mathiesen G, Eijsink VG. Expression of endoglucanases in Pichia pastoris under control of the GAP promoter. Microb Cell Fact. 2014;13(1):57.
|
Human antithrombin (rAT) |
RH101 |
AOX2 |
Methanol |
25 ºC |
0.1 mg ml-1
|
6262 Kuwae S, Ohyama M, Ohya T, Ohi H, Kobayashi K. Production of recombinant human antithrombin by Pichia pastoris. J Biosci Bioeng. 2005;99(3):264-271.
|
B-cell maturation antigen |
GS115 |
AOX1 |
Methanol |
30 ºC |
0.207 mg ml-1
|
6363 Hou W, Meng X, Wang Y, Mo W, Wu Y, Yu M. Characterization and high-yield production of non-N-glycosylated recombinant human BCMA-Fc in Pichia pastoris. Eng Life Sci. 2016, http://dx.doi.org/10.1002/elsc.201600039. http://dx.doi.org/10.1002/elsc.201600039...
|
Fungal immunomodulatory protein |
GS115 |
AOX1 |
Methanol |
25 ºC |
0.149 mg ml-1
|
6464 Lin J-W, Guan S-Y, Duan Z-W, et al. Gene cloning of a novel fungal immunomodulatory protein from Chroogomphis rutilus and its expression in Pichia pastoris. J Chem Technol Biotechnol. 2016;91:2761-2768.
|
Human alpha-defensin 5 |
GS115 |
AOX1 |
Methanol |
30 ºC |
0.165 mg ml-1
|
6565 Wang Y, Xuan Y, Zhang P, et al. Targeting expression of the catalytic domain of the kinase insert domain receptor (KDR) in the peroxisomes of Pichia pastoris. FEMS Yeast Res. 2009;9(5):732-741.
|