SciELO - Scientific Electronic Library Online

 
vol.4 issue1Relationship between muscular strength and flexibility in healthy adults of both gendersThe use of anabolic-androgenic steroids by weight lifters and physical fitness athletes author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand

Journal

Article

Indicators

Related links

Share


Revista Brasileira de Medicina do Esporte

Print version ISSN 1517-8692

Abstract

LATORRACA, Márcia Queiroz et al. Description of purified diet to induce protein malnutrition in rats. Rev Bras Med Esporte [online]. 1998, vol.4, n.1, pp.9-12. ISSN 1517-8692.  http://dx.doi.org/10.1590/S1517-86921998000100003.

The present work describes the composition of a purified low-protein diet (6% protein) formulated to produce protein malnutrition in rodents. The diet utilized in laboratory was based on the AIN-93 (report of the American Institute of Nutrition establishing dietary standards for nutritional studies with laboratory rodents), which was modified to investigate in malnourished rats the metabolic alterations induced by protein-deprivation associated to situations as physical exercise, pregnancy and diabetes. This diet has the following composition (g/Kg): cornstarch (480), casein (71,5), dextrinized cornstarch (159), sucrose (121), soybean oil (70), microcellulose (50), mineral mix-AIN-93-G-Mx (35), vitamin mix-AIN-93-Vx (10), L-cystine (1), choline chlorhydrate (2,5). Rats fed such diet showed features of malnutrition which have been observed in man and laboratory animals: low weight gain rates, low serum protein and albumin levels, high serum free fatty acids and increase of hepatic glycogen stores.

Keywords : Purified low-protein diet; Protein malnutrition; Rodents.

        · abstract in Portuguese     · text in Portuguese     · Portuguese ( pdf epdf )

 

Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License