Ethylene synthesis inhibition effects on oxidative stress and
<i>in vitro</i> conservation of <i>Lippia filifolia</i>
(Verbenaceae)

Pimenta, MR.; Ribeiro, C.; Soares, CQG.; Mendes, GC.; Braga, VF.; Reis, LB.; Otoni, WC.; Resende, CF.; Viccini, LF.; Peixoto, PHP.

doi:10.1590/S1519-69842013000300020

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Brazilian Journal of Biology

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Biology • Braz. J. Biol. 73 (3) • Aug 2013 • https://doi.org/10.1590/S1519-69842013000300020 copy

Ethylene synthesis inhibition effects on oxidative stress and in vitro conservation of Lippia filifolia (Verbenaceae)

Efeitos da Inibição da síntese do etileno no estresse oxidativo e na conservação in vitro de Lippia filifolia (Verbenaceae)

Authorship SCIMAGO INSTITUTIONS RANKINGS

This study aimed to investigate the effects of ethylene biosynthesis inhibitors on oxidative metabolisms and the in vitro conservation of Lippia filifolia, using the lipid peroxidation index (TBARS), antioxidative enzymes and pigments as biomarkers. We found that EDTA, sodium thiosulfate (STS) and especially Co had protective effects on oxidative stress in tissues cultured in vitro, resulting in a delay of the senescence and the reduction of subcultures frequency, con-tributing to the germplasm conservation of this species.

ethylene inhibitors; TBARS; ROS; micropropagation; germplasm conservation

Treatment	TBAR	SOD	POD	PPO	CAT	Chl a + b	Carot.	Chla / Chlb	Anthoc.
Control	0.607	0.499	0.157	0.030	1.436	0.573	0.185	2.981	3.740
AgNO₃ 6 µM	1.003 c⁺	0.721 b⁼	0.209 b⁼	0.071 c⁺	1.792 b⁼	0.303 a^–	0.212 a⁼	0.302 b^–	0.995 b^–
AgNO₃ 12 µM	1.906 b⁺	1.459 a⁺	0.534 a⁺	0.205 a⁺	4.514 a⁺	0.230 b^–	0.153 b-	0.352 b^–	1.800 a^–
AgNO₃ 18 µM	2.931 a⁺	0.573 b⁼	0.554 a⁺	0.159 b⁺	3.720 a⁺	0.207 b^–	0.127 c^–	0.580 a^–	1.656 a^–
SA 80 µM	1.005 a⁺	0.505 a⁼	0.246 c⁺	0.087 c⁺	4.836 a⁺	0.235 b^–	0.216 a⁺	0.285 b^–	4.020 a⁼
SA 160 µM	0.672 a⁼	0.793 a⁺	0.324 a⁺	0.127 a⁺	3.504 b⁺	0.267 a^–	0.220 a⁺	0.427a^–	2.933 b^–
SA 240 µM	0.476 b^–	0.695 a⁼	0.272 b⁺	0.108 b⁺	3.618 b⁺	0.283 a^–	0.209 a⁺	0.515a^–	3.454 b^–
Co 20 µM	0.116 a^–	0.266 b^–	0.082 b^–	0.020 c^–	1.091 b^–	0.138 a^–	0.105 a^–	1.625 b^–	2.025 a^–
Co 40 µM	0.070 a^–	0.403 a^–	0.163 a⁼	0.045 a⁺	1.432 a⁼	0.092 b^–	0.055 b^–	1.377 b^–	2.480 a^–
Co 60 µM	0.072 a^–	0.241 b^–	0.128 a⁼	0.034 b⁼	1.204 b^–	0.035 c^–	0.033 b^–	3.121 a⁼	0.529 b^–
EDTA 45 µM	0.313 a^–	0.519 a⁼	0.237 a⁺	0.055 a⁺	2.410 a⁺	0.082 b^–	0.052 a^–	3.473 a⁺	2.535 b^–
EDTA 90 µM	0.165 b^–	0.317 b^–	0.200 b⁺	0.041 b⁺	1.672 b⁺	0.095 a^–	0.039 b^–	1.555 b^–	5.013 a⁺
EDTA 135 µM	0.152 b^–	0.370 a⁼	0.143 c⁼	0.037 b⁼	1.718 b⁺	0.085 b^–	0.035 b^–	1.939 b^–	3.929 a⁼
STS 6 µM	0.286 a^–	0.973 a⁺	0.268 a⁺	0.068 a⁺	2.300 a⁺	0.173 a^–	0.042 b^–	1.912 c^–	2.621 b^–
STS 12 µM	0.268 a^–	0.560 b⁼	0.232 b⁺	0.067 a⁺	2.522 a⁺	0.200 a^–	0.048 b^–	3.383 b⁼	2.653 b^–
STS 18 µM	0.216 a^–	0.203 c^–	0.156 c⁼	0.041 b⁺	1.206 b^–	0.186 a^–	0.084 a^–	4.051 a⁺	5.079 a⁼

Instituto Internacional de Ecologia R. Bento Carlos, 750, 13560-660 São Carlos SP - Brasil, Tel. e Fax: (55 16) 3362-5400 - São Carlos - SP - Brazil
E-mail: bjb@bjb.com.br

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[1] *e-mail: paulo.peixoto@ufjf.edu.br