SciELO - Scientific Electronic Library Online

 
vol.76 número1Distribution of trace elements in tissues of shrimp species Litopenaeus vannamei (Boone, 1931) from Bahia, BrazilFish hatchering and its effects on the morphology of Prochilodus lineatus (Actinopterygii: Prochilodontidae) índice de autoresíndice de materiabúsqueda de artículos
Home Pagelista alfabética de revistas  

Servicios Personalizados

Revista

Articulo

Indicadores

Links relacionados

Compartir


Brazilian Journal of Biology

versión impresa ISSN 1519-6984versión On-line ISSN 1678-4375

Resumen

MORA, N. H. A. P. et al. Polymorphism C242T in the Cyp19 gene in meat sheep. Braz. J. Biol. [online]. 2016, vol.76, n.1, pp.205-208.  Epub 22-Ene-2016. ISSN 1519-6984.  http://dx.doi.org/10.1590/1519-6984.17414.

The objective of this study was to evaluate the frequency of C242T polymorphism on the aromatase gene and the allelic and genotypic frequency of these variants in sheep belonging to four breed groups. Blood samples were collected from 187 animals of four breed groups: Dorper, Santa Inês, Texel and White Dorper, originated from herds in the region of Maringá/PR, Brazil. The genomic DNA was extracted using alkaline extraction, with subsequent amplification of the fragments via PCR with specific primer. The samples resulting from amplification were subjected to digestion process using the Dpn II restriction enzyme and to polyacrylamide gel electrophoresis 10.0% and stained with silver nitrate. Three distinct genotypes were observed: homozygous (CC), heterozygous (CT) and homozygous for no cut (TT). The resulting data were analyzed using the POPGENE software with 5% significance. Genotypic frequencies among the breed groups were: Texel (CC - 0.426; CT - 0.511; TT - 0.064), Dorper (CC - 0.073; CT - 0.732; TT - 0.439), White Dorper (CC - 0.021; CT - 0.255; TT - 0.723) and Santa Inês (CC - 0.115; CT - 0.462; TT - 0.423).

Palabras clave : Cyp19; Dorper; polymorphism; Santa Inês; Texel; White Dorper.

        · resumen en Portugués     · texto en Inglés     · Inglés ( pdf )