Proline levels, oxidative metabolism and photosynthetic pigments during <i>in vitro</i> growth and acclimatization of <i>Pitcairnia encholirioides</i> L.B. Sm. (Bromeliaceae)

Resende, C. F.; Braga, V. F.; Pereira, P. F.; Silva, C. J.; Vale, V. F.; Bianchetti, R. E.; Forzza, R. C.; Ribeiro, C.; Peixoto, P. H. P.

doi:10.1590/1519-6984.19314

C. F. Resende ^**e-mail: cristianoig2004@hotmail.com

Laboratório de Fisiologia Vegetal, Departamento de Botânica, Instituto de Ciências Biológicas, Universidade Federal de Juiz de Fora – UFJF, Campus Universitário, Bairro Martelos, CEP 36036-900, Juiz de Fora, MG, Brazil

V. F. Braga

Laboratório de Fisiologia Vegetal, Departamento de Botânica, Instituto de Ciências Biológicas, Universidade Federal de Juiz de Fora – UFJF, Campus Universitário, Bairro Martelos, CEP 36036-900, Juiz de Fora, MG, Brazil

P. F. Pereira

Laboratório de Fisiologia Vegetal, Departamento de Botânica, Instituto de Ciências Biológicas, Universidade Federal de Juiz de Fora – UFJF, Campus Universitário, Bairro Martelos, CEP 36036-900, Juiz de Fora, MG, Brazil

C. J. Silva

Laboratório de Fisiologia Vegetal, Departamento de Botânica, Instituto de Ciências Biológicas, Universidade Federal de Juiz de Fora – UFJF, Campus Universitário, Bairro Martelos, CEP 36036-900, Juiz de Fora, MG, Brazil

V. F. Vale

Laboratório de Fisiologia Vegetal, Departamento de Botânica, Instituto de Ciências Biológicas, Universidade Federal de Juiz de Fora – UFJF, Campus Universitário, Bairro Martelos, CEP 36036-900, Juiz de Fora, MG, Brazil

R. E. Bianchetti

Laboratório de Fisiologia Vegetal, Departamento de Botânica, Instituto de Ciências Biológicas, Universidade Federal de Juiz de Fora – UFJF, Campus Universitário, Bairro Martelos, CEP 36036-900, Juiz de Fora, MG, Brazil

R. C. Forzza

Instituto de Pesquisa do Jardim Botânico do Rio de Janeiro, Rua Pacheco Leão, 915, CEP 22460-030, Rio de Janeiro, RJ, Brazil

C. Ribeiro

Departamento de Biologia Geral, Centro de Ciências Biológicas, Universidade Federal de Viçosa – UFV, CEP 36571-000, Viçosa, MG, Brazil

P. H. P. Peixoto

Laboratório de Fisiologia Vegetal, Departamento de Botânica, Instituto de Ciências Biológicas, Universidade Federal de Juiz de Fora – UFJF, Campus Universitário, Bairro Martelos, CEP 36036-900, Juiz de Fora, MG, Brazil

*e-mail: cristianoig2004@hotmail.com

Figures (1)
Tables (4)

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Figure 1
Pitcairnia encholirioides L.B. Sm. plantlets achieved after 150 d of in vitro culture (a), after 50 d (b) and 120 d (c) of acclimatization in a greenhouse. Treatments: (1) 10 µM GA₃, 15 g L^–1 sucrose, unsealed closure; (2) 10 µM GA₃, 15 g L^–1 sucrose, sealed closure; (3) 10 µM GA₃, 30 g L^–1 sucrose, unsealed closure; (4)10 µM GA3, 30 g L–1 sucrose, sealed closure; (5) 0.2 µM NAA, 15 g L^–1 sucrose, unsealed closure; (6) 0.2 µM NAA, 15 g L^–1 sucrose, sealed closure; (7) 0.2 µM NAA, 30 g L^–1 sucrose, unsealed closure; and 8) 0.2 µM NAA, 30 g L^–1 sucrose, sealed closure. Scale bar: 1 cm.

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Table 1
Proline [μmol g^–1 (f.m.)] contents and SOD [U mg^–1 (prot.)], CAT [mmol mg^–1 (prot.) min^–1], POD [μmol mg^–1 (prot.) min^–1] and PPO [μmol mg^–1 (prot.) min^–1] activities in leaf tissues of Pitcairnia encholirioides after 150 days of in vitro culture in presence of GA₃ or NAA, in two sucrose concentrations (15 or 30 g L^–1) and under two types of closure (unsealed or sealed closure - US or S).

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Table 2
Proline [μmol g^-1 (f.m.)] contents and SOD [U mg^-1 (prot.)], CAT [mmol mg^–1 (prot.) min^–1], POD [μmol mg^–1 (prot.) min^–1] and PPO [μmol mg^–1 (prot.) min^–1] activities in leaf tissues of Pitcairnia encholirioides, initially cultured in vitro for 150 d in the presence of GA₃ or NAA, in two sucrose concentrations (15 or 30 g L^–1) and under two types of closure (unsealed or sealed closure - US or S), 180 d after acclimatization.

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Table 3
Chlorophyll a [mg g^-1 (f.m.)], chlorophyll b [mg g^-1 (f.m.)], chlorophyll a + b [mg g^-1 (f.m.)] and carotenoids [mg g^-1 (f.m.)] contents, chlorophyll a/b ratio and chlorophyll a + b/carotenoids ratio in leaf tissues of Pitcairnia encholirioides after 150 d of in vitro culture in presence of GA₃ or NAA, in two sucrose concentrations (15 or 30 g L^-1) and under two types of closure (unsealed closure or sealed - US or S).

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Table 4
Chlorophyll a [mg g^–1 (f.m.)], chlorophyll b [mg g^–1 (f.m.)], chlorophyll a + b [mg g^–1 (f.m.)] and carotenoids [mg g^–1 (f.m.)] contents, chlorophyll a/b ratio and chlorophyll a + b/carotenoids ratio in leaf tissues of Pitcairnia encholirioides, initially cultured in vitro for 150 d in presence of GA₃ or NAA, in two sucrose concentrations (15 or 30 g L^–1) and under two types of closure (unsealed closure or sealed - US or S), 180 d after acclimatization.

Treatment	Proline	SOD	CAT	POD	PPO
GA₃ US 15	1.200 Aa¹ 1 In each growth regulator (GA3 or NAA), means identified by capital letters compare the effects of sucrose concentration for each type of closure, and means identified by lower case letters compare the effects of the type of closure used in vitro at each sucrose concentration. Means identified by the same uppercase or lowercase letters do not differ significantly by the Scott-Knott test at 5% probability.	304.22 Aa	0.744 Aa	81.236 Ba	25.157 Aa
GA₃ US 30	1.124 Aa	189.09 Bb	0.583 Ba	97.658 Aa	26.523 Aa
GA₃ S 15	1.027 Ab	353.92 Aa	0.785 Aa	84.905 Ba	20.483 Ab
GA₃ S 30	0.925 Ab	257.83 Ba	0.627 Ba	93.248 Aa	20.000 Ab
NAA US 15	1.008 Ba	261.33 Ab	0.911 Ab	68.303 Ba	17.321 Aa
NAA US 30	1.166 Ab	200.57 Ba	0.683 Bb	91.902 Aa	11.432 Ba
NAA S 15	1.093 Ba	358.77 Aa	1.160 Aa	74.779 Aa	13.815 Ab
NAA S 30	1.447 Aa	222.81 Ba	0.822 Ba	81.874 Ab	10.140 Bb

Treatment	Proline	SOD	CAT	POD	PPO
GA₃ US 15	1.425 A¹ 1 Means identified by the same letters (comparing the effects of sucrose concentration, in each growth regulator, for each type of closure) do not differ significantly by the Scott-Knott test at 5% probability.	132.04 A	0.994 A	79.383 B	34.842 A
GA₃ US 30	1.309 A	240.21 A	1.231 A	95.207 A	32.562 A
GA₃ S 15	1.193 A	291.43 A	1.232 B	112.959 A	52.356 A
GA₃ S 30	0.914 A	312.75 A	1.962 A	126.952 A	44.273 A
NAA US 15	0.990 A	285.36 A	1.939 A	98.739 A	35.759 A
NAA US 30	1.375 A	350.14 A	1.509 B	101.602 A	35.185 A
NAA S 15	0.845 A	241.28 A	2.504 A	145.465 A	50.327 A
NAA S 30	1.147 A	301.27 A	1.568 B	95.525 B	41.461 B

Treatment	Chl a	Chl b	Chl a + b	Carot	Chl a/Chl b	Chl a + b/ Carot
GA₃ US 15	0.912 Aa¹	0.343 Aa	1.256 Aa	0.284 Aa	2.651 Aa	4.415 Aa
GA₃ US 30	1.034 Aa	0.354 Ab	1.388 Aa	0.333 Aa	2.999 Aa	4.200 Ab
GA₃ S 15	0.671 Bb	0.254 Ba	0.957 Bb	0.203 Bb	2.645 Aa	4.560 Aa
GA₃ S 30	1.222 Aa	0.490 Aa	1.713 Aa	0.359 Aa	2.506 Aa	4.765 Aa
NAA US 15	0.678 Aa	0.281 Aa	0.960 Aa	0.186 Aa	2.407 Aa	5.141 Aa
NAA US 30	0.408 Ba	0.212 Ba	0.621 Ba	0.128 Ba	1.922 Bb	4.843 Bb
NAA S 15	0.298 Ab	0.220 Ab	0.737 Ab	0.144 Ab	2.345 Aa	5.122 Aa
NAA S 30	0.273 Bb	0.133 Bb	0.407 Bb	0.079 Bb	2.042 Ba	5.135 Aa

Treatment	Chl a	Chl b	Chl a + b	Carot	Chl a/Chl b	Chl a + b/ Carot
GA₃ US 15	0.385 A¹ 1 Means identified by the same letters (comparing the effects of sucrose concentration, in each growth regulator, for each type of closure) do not differ significantly by the Scott-Knott test at 5% probability.	0.141 A	0.527 A	0.144 A	2.723 A	3.652 A
GA₃ US 30	0.354 A	0.126 A	0.480 A	0.139 A	2.822 A	3.457 A
GA₃ S 15	0.356 A	0.130 A	0.486 A	0.139 A	2.794 A	3.450 A
GA₃ S 30	0.293 A	0.107 A	0.400 A	0.108 A	2.711 A	3.718 A
NAA US 15	0.454 A	0.161 A	0.616 A	0.171 A	2.812 B	3.595 A
NAA US 30	0.360 A	0.107 A	0.468 A	0.132 A	3.448 A	3.535 A
NAA S 15	0.398 A	0.132 A	0.531 A	0.145 A	3.022 A	3.630 A
NAA S 30	0.368 A	0.138 A	0.507 A	0.151 A	2.677 A	3.344 A

[1] *e-mail: cristianoig2004@hotmail.com

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Brasil

Proline levels, oxidative metabolism and photosynthetic pigments during in vitro growth and acclimatization of Pitcairnia encholirioides L.B. Sm. (Bromeliaceae)

Niveis de prolina, do metabolismo oxidativo e dos pigmentos fotossintéticos durante o crescimento in vitro e aclimatização de Pitcairnia encholirioides L.B. Sm. (Bromeliaceae)

Abstract