Responses of antioxidant enzymes, photosynthetic pigments and carbohydrates in micropropagated <i>Pitcairnia encholirioides</i> L.B. Sm. (Bromeliaceae) under <i>ex vitro</i> water deficit and after rehydration

Resende, C. F.; Pacheco, V. S.; Dornellas, F. F.; Oliveira, A. M. S.; Freitas, J. C. E.; Peixoto, P. H. P.

doi:10.1590/1519-6984.175284

Acessibilidade / Reportar erro

Brasil

Brazilian Journal of Biology

Español English

Brasil

Español English

sumário « anterior atual seguinte »

Sumário

Original Article • Braz. J. Biol. 79 (1) • Jan-Mar 2019 • https://doi.org/10.1590/1519-6984.175284 copy

Responses of antioxidant enzymes, photosynthetic pigments and carbohydrates in micropropagated Pitcairnia encholirioides L.B. Sm. (Bromeliaceae) under ex vitro water deficit and after rehydration

Respostas de enzimas antioxidantes, pigmentos fotossintéticos e carboidratos em Pitcairnia encholirioides L.B. Sm. (Bromeliaceae) micropropagadas, sob déficit hídrico ex vitro e após reidratação

Authorship SCIMAGO INSTITUTIONS RANKINGS

Abstract

In this study, the activities of antioxidant enzymes, photosynthetic pigments, proline and carbohydrate contents in Pitcairnia encholirioides under ex vitro conditions of water deficit were evaluated. Results show that plants under progressive water stress, previously in vitro cultured in media supplemented with 30 g L^-1 sucrose and GA₃, accumulated more proline and increased peroxidase (POD) activity and the contents of photosynthetic pigments and carbohydrates. For plants previously in vitro cultured with 15 g L^-1 sucrose and NAA, no differences were found for proline content and there were reductions in activities of peroxidase (POD), catalase (CAT) and poliphenoloxidase (PPO), and in contents of carbohydrates, with progress of ex vitro water deficit. After rehydration, plants showed physiological recovery, with enzymatic activities and contents of metabolites similar to those found in the controls not submitted to dehydration, regardless of the previous in vitro culture conditions. These results show that micropropagated P. encholirioides has high tolerance to dehydration once in ex vitro conditions, which can ensure the survival of plants from tissue culture when transferred to its natural environment, emphasizing the importance of such biotechnology for the propagation of endangered species.

Keywords:
antioxidant enzymes; carbohydrates; photosynthetic pigments; proline; water stress

Treatment		Total proteins	Proline	SOD	CAT	POD	PPO
GA₃ 30 g L^-1	0	0.097 ± 0.004 B	0.97 ± 0.09 B	261.8 ± 25.7 A	1.45 ± 0.05 A	70.2 ± 6.3 C	34.2 ± 1.5 A
	30	0.106 ± 0.003 B	1.21 ± 0.12 B	201.7 ± 14.5 B	1.46 ± 0.07 A	115.1 ± 3.6 B	35.9 ± 2.1 A
	42	0.218 ± 0.005 A	1.48 ± 0.05 A	168.7 ± 16.3 B	1.49 ± 0.01 A	251.3 ± 6.9 A	31.4 ± 0.6 B
	54	0.220 ± 0.004 A	1.58 ± 0.27 A	165.5 ± 12.0 B	1.48 ± 0.03 A	258.3 ± 12.8 A	29.8 ± 0.6 B
NAA 15 g L^-1	0	0.085 ± 0.003 C	0.96 ± 0.20 A	141.2 ± 18.1 A	3.05 ± 0.02 A	217.2 ± 5.9 A	49.5 ± 1.4 A
	30	0.146 ± 0.001 B	1.11 ± 0.13 A	114.2 ± 9.8 A	1.93 ± 0.08 B	223.3 ± 8.6 A	39.0 ± 2.9 B
	42	0.142 ± 0.003 B	1.20 ± 0.17 A	117.1 ± 6.5 A	1.80 ± 0.08 B	161.9 ± 4.0 B	34.4 ± 1.5 B
	54	0.198 ± 0.009 A	1.17 ± 0.05 A	115.8 ± 8.4 A	1.72 ± 0.16 B	167.1 ± 9.3 B	27.1 ± 0.7 C

Treatment		Total proteins	Proline	SOD	CAT	POD	PPO
GA₃ 30 g L^-1	0	0.095 ± 0.00 A	1.08 ± 0.20 A	105.6 ± 18.2 A	0.64 ± 0.04 A	87.2 ± 2.0 A	58.3 ± 2.6 A
	30	0.102 ± 0.00 A	1.21 ± 0.24 A	110.2 ± 6.2 A	0.66 ± 0.03 A	82.5 ± 2.8 A	58.5 ± 2.7 A
	42	0.099 ± 0.00 A	1.23 ± 0.19 A	102.3 ± 3.6 A	0.66 ± 0.06 A	85.5 ± 3.0 A	59.5 ± 7.8 A
	54	0.091 ± 0.00 A	1.18 ± 0.30 A	102.6 ± 18.4 A	0.67 ± 0.03 A	88.1 ± 7.1 A	62.2 ± 3.1 A
NAA 15 g L^-1	0	0.103 ± 0.00 A	1.24 ± 0.20 A	131.8 ± 30.0 A	0.60 ± 0.08 A	81.9 ± 6.0 A	45.1 ± 2.2 A
	30	0.107 ± 0.00 A	1.25 ± 0.50 A	127.6 ± 2.4 A	0.58 ± 0.04 A	84.0 ± 5.0 A	44.9 ± 1.4 A
	42	0.110 ± 0.01 A	1.16 ± 0.17 A	128.5 ± 3.0 A	0.57 ± 0.02 A	83.6 ± 14.2 A	44.8 ± 4.4 A
	54	0.113 ± 0.00 A	1.31 ± 0.23 A	126.7 ± 7.6 A	0.59 ± 0.05 A	79.6 ± 2.3 A	47.4 ± 3.6 A

Treatment		Chl a	Chl b	Chl a + b	Carot	Chl a/b	Chl a + b/ Carot
GA₃ 30 g L^-1	0	0.45 ± 0.04 B	0.19 ± 0.02 B	0.64 ± 0.06 B	0.14 ± 0.01 B	2.38 ± 0.03 A	4.34 ± 0.08 A
	30	0.47 ± 0.02 B	0.19 ± 0.01 B	0.66 ± 0.03 B	0.15 ± 0.00 B	2.40 ± 0.02 A	4.38 ± 0.04 A
	42	0.89 ± 0.02 A	0.40 ± 0.01 A	1.29 ± 0.03 A	0.33 ± 0.01 A	2.19 ± 0.03 B	3.87 ± 0.03 B
	54	1.02 ± 0.07 A	0.47 ± 0.03 A	1.49 ± 0.10 A	0.38 ± 0.02 A	2.16 ± 0.02 B	3.88 ± 0.05 B
NAA 15 g L^-1	0	0.38 ± 0.02 C	0.16 ± 0.01 C	0.54 ± 0.03 C	0.12 ± 0.00 D	2.36 ± 0.00 A	4.56 ± 0.00 A
	30	0.73 ± 0.07 A	0.34 ± 0.02 A	1.07 ± 0.10 A	0.28 ± 0.01 A	2.11 ± 0.07 B	3.78 ± 0.16 B
	42	0.48 ± 0.00 B	0.23 ± 0.00 B	0.71 ± 0.00 B	0.17 ± 0.00 C	2.12 ± 0.01 B	4.15 ± 0.14 B
	54	0.69 ± 0.02 A	0.33 ± 0.01 A	1.02 ± 0.03 A	0.25 ± 0.00 B	2.09 ± 0.00 B	4.00 ± 0.10 B

Treatment		Chl a	Chl b	Chl a + b	Carot	Chl a/b	*Chl a + b/ Carot*
GA₃ 30 g L^-1	0	0.39 ± 0.02 A	0.15 ± 0.01 B	0.54 ± 0.03 A	0.13 ± 0.00 A	2.52 ± 0.01 A	4.19 ± 0.02 C
	30	0.47 ± 0.01 A	0.20 ± 0.00 A	0.67 ± 0.03 A	0.15 ± 0.00 A	2.35 ± 0.01 B	4.33 ± 0.04 B
	42	0.51 ± 0.10 A	0.22 ± 0.05 A	0.73 ± 0.15 A	0.16 ± 0.03 A	2.33 ± 0.04 B	4.50 ± 0.02 A
	54	0.58 ± 0.01 A	0.25 ± 0.00 A	0.83 ± 0.02 A	0.18 ± 0.00 A	2.34 ± 0.04 B	4.47 ± 0.04 A
NAA 15 g L^-1	0	0.52 ± 0.00 B	0.22 ± 0.00 B	0.75 ± 0.00 B	0.16 ± 0.00 B	2.31 ± 0.04 B	4.52 ± 0.17 A
	30	0.51 ± 0.03 B	0.20 ± 0.01 C	0.71 ± 0.04 B	0.16 ± 0.01 B	2.51 ± 0.04 A	4.39 ± 0.05 A
	42	0.55 ± 0.04 B	0.25 ± 0.02 B	0.80 ± 0.07 B	0.18 ± 0.01 B	2.28 ± 0.03 B	4.49 ± 0.04 A
	54	0.65 ± 0.00 A	0.29 ± 0.00 A	0.94 ± 0.00 A	0.21 ± 0.00 A	2.20 ± 0.05 C	4.48 ± 0.11 A

Treatment		TSS	RS	Sucrose	Starch
GA₃ 30 g L^-1	0	25.95 ± 0.25 C	16.30 ± 0.48 C	9.65 ± 0.77 B	27.84 ± 1.07 A
	30	25.67 ± 0.83 C	16.02 ± 0.55 C	9.64 ± 0.53 B	28.02 ± 1.70 A
	42	29.25 ± 0.43 B	19.79 ± 0.53 B	9.45 ± 0.33 B	28.94 ± 0.28 A
	54	37.76 ± 1.05 A	28.49 ± 1.26 A	11.74 ± 0.73 A	30.27 ± 1.12 A
NAA 15 g L^-1	0	27.50 ± 1.98 B	19.15 ± 1.05 B	8.35 ± 1.10 B	27.34 ± 1.75 C
	30	35.28 ± 1.43 A	23.82 ± 1.53 A	10.46 ± 1.17 A	31.45 ± 1.16 C
	42	36.58 ± 2.54 A	24.95 ± 2.09 A	11.63 ± 0.10 A	43.93 ± 4.21 B
	54	40.64 ± 0.68 A	28.49 ± 2.60 A	12.14 ± 0.80 A	58.04 ± 1.08 A

Treatment		TSS	RS	Sucrose	Starch
GA₃ 30 g L^-1	0	7.41 ± 0.55 A	2.44 ± 0.64 A	4.97 ± 0.20 A	18.65 ± 3.27 A
	30	7.43 ± 0.68 A	2.42 ± 0.85 A	5.00 ± 0.51 A	19.04 ± 0.61 A
	42	7.67 ± 0.36 A	2.46 ± 0.93 A	5.21 ± 0.76 A	19.36 ± 1.23 A
	54	8.08 ± 0.15 A	2.49 ± 0.17 A	5.59 ± 0.04 A	19.23 ± 0.56 A
NAA 15 g L^-1	0	10.22 ± 1.04 A	2.93 ± 0.85 A	7.24 ± 0.38 B	25.95 ± 0.26 A
	30	10.09 ± 0.27 A	2.45 ± 0.32 A	7.64 ± 0.33 B	27.11 ± 2.04 A
	42	9.81 ± 0.31 A	1.52 ± 0.30 A	8.29 ± 0.04 A	27.46 ± 2.03 A
	54	10.03 ± 0.51 A	1.34 ± 0.83 A	8.69 ± 0.36 A	28.13 ± 0.45 A

Instituto Internacional de Ecologia R. Bento Carlos, 750, 13560-660 São Carlos SP - Brasil, Tel. e Fax: (55 16) 3362-5400 - São Carlos - SP - Brazil
E-mail: bjb@bjb.com.br

Acompanhe os números deste periódico no seu leitor de RSS

[1] *e-mail: cristianoig2004@hotmail.com