Powdered coconut water as preservant of semi-heavy cocks semen

Freitas, Brenna Kelen Mello de; Cruz, Frank George Guimarães; Rufino, João Paulo Ferreira; Feijó, Julmar da Costa; Melo, Ramon Duque; Melo, Lucas Duque

doi:10.1590/S1519-99402018000200007

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Reprodução Animal • Rev. bras. saúde prod. anim. 19 (2) • Apr-Jun 2018 • https://doi.org/10.1590/S1519-99402018000200007 copy

Powdered coconut water as preservant of semi-heavy cocks semen

Água de coco como preservante de sêmen de galos semipesados

Authorship SCIMAGO INSTITUTIONS RANKINGS

SUMMARY

This study aimed to evaluate the effect of powered coconut water as preservant of cocks semen in different times. The experimental method was completely randomized, with treatments constituted by different times (1, 5, 10 and 15 minutes) of powered coconut water as preservant of cocks semen (Rhode Island Red lineage with 30-weeks). After collect and use of powered coconut water as preservant, the semen samples were used for inseminated of breeders of same lineage and age (16 per treatment). 280 eggs were collected for evaluation of effects on chicks (70 eggs per treatment). Were evaluated the incubation yields, chick/egg relation and gastrointestinal tract development of chicks. The data collected were submitted for variance analysis and evaluated by Tukey test at 5% of significance. Differences (p<0.05) were observed in incubation yields, chick/egg relation and gastrointestinal tract development, with the exception (p>0.05) of oropharynx + oesophagus length and yolk sac weight. From these results, it was concluded that the powered coconut water can be used as preservation of cocks semen up to 15 minutes. Cells exposed more time to nutrients provided by the powdered coconut water showed eggs and chicks most heavier, and better results in the incubation yields and embryo development.

Keywords:
biotechnology; embryo development; fertility; preservation

Semen preservation times	Variables
Semen preservation times	Egg weight (g)	Egg weight loss (%)	Chick weight (g)	Chick/egg correlation
1 minute	49.84^b	8.48^bc	30.54^b	0.67^b
5 minutes	50.36^ab	9.65^b	0.00^c	0.00^c
10 minutes	50.40^ab	10.82^ab	32.84^ab	0.73^ab
15 minutes	50.54^a	12.03^a	33.50^a	0.75^a
p-value	0.05	0.01	0.01	0.01
Effect	^* * Means followed by lowercase letters in column differ in 5% by Tukey test (P<0.05).	^* * Means followed by lowercase letters in column differ in 5% by Tukey test (P<0.05).	^* * Means followed by lowercase letters in column differ in 5% by Tukey test (P<0.05).	^* * Means followed by lowercase letters in column differ in 5% by Tukey test (P<0.05).
CV (%)	1.52	11.37	11.27	11.70

Semen preservation times	Variables
Semen preservation times	Fertility (%)	Hatchability (%)	Hatching (%)
1 minute	67.14^b	46.72^b	31.43^b
5 minutes	0.00^c	0.00^c	0.00^c
10 minutes	41.43^ab	79.28^ab	32.86^ab
15 minutes	75.71^a	86.64^a	65.71^a
p-value	0.02	0.01	0.01
Effect	^* * Means followed by lowercase letters in column differ in 5% by Tukey test (P<0.05); ns = non significant.	^* * Means followed by lowercase letters in column differ in 5% by Tukey test (P<0.05); ns = non significant.	^* * Means followed by lowercase letters in column differ in 5% by Tukey test (P<0.05); ns = non significant.
CV (%)	9.56	4.02	14.25

Semen cryopreservation times	Variables
Semen cryopreservation times	Yolk sac (g)	Heart (g)	Liver (g)	Pancreas (g)	Pro-ventricule (g)	Gizzard (g)
1 minute	2.38	0.62^ab	1.37^ab	0.12^bc	0.61^ab	2.23^bc
10 minutes	2.34	0.61^ab	1.48^ab	0.31^ab	0.63^ab	2.31^ab
15 minutes	2.29	0.82^a	1.74^a	0.42^a	0.78^a	2.55^a
p-value	0.90	0.01	0.01	0.02	0.04	0.03
Effect	ns	^* * Means followed by lowercase letters in column differ in 5% by Tukey test (P<0.05); ns = non significant.	^* * Means followed by lowercase letters in column differ in 5% by Tukey test (P<0.05); ns = non significant.	^* * Means followed by lowercase letters in column differ in 5% by Tukey test (P<0.05); ns = non significant.	^* * Means followed by lowercase letters in column differ in 5% by Tukey test (P<0.05); ns = non significant.	^* * Means followed by lowercase letters in column differ in 5% by Tukey test (P<0.05); ns = non significant.
CV (%)	17.23	17.28	14.63	14.77	10.49	19.16

Semen preservation times	Variables
Semen preservation times	Gastrointestinal tract (cm)	Oropharynx + oesophagus (cm)	Duodenal loop (cm)	Jejunum + ileum (cm)	Cecum (cm)	Colon + rectum (cm)
1 minute	43.06^b	6.60	4.72^b	23.98^b	3^	7.70^b
10 minutes	48.20^ab	6.16	5.80^ab	24.00^ab	4.50^ab	9.00^a
15 minutes	53.40^a	6.70	6.40^a	30.20^a	4.70^a	9.00^a
p-value	0.01	0.37	0.05	0.03	0.03	0.02
Effect	^* * Means followed by lowercase letters in column differ in 5% by Tukey test (P<0.05); ns = non significant.	ns	^* * Means followed by lowercase letters in column differ in 5% by Tukey test (P<0.05); ns = non significant.	^* * Means followed by lowercase letters in column differ in 5% by Tukey test (P<0.05); ns = non significant.	^* * Means followed by lowercase letters in column differ in 5% by Tukey test (P<0.05); ns = non significant.	^* * Means followed by lowercase letters in column differ in 5% by Tukey test (P<0.05); ns = non significant.
CV (%)	9.27	9.62	8.29	14.65	14.32	16.78

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