A list of aquatic and semi-aquatic Heteroptera species from the southern region of Mato Grosso do Sul State is presented. The material was collected between 2008 and 2009. Samples were obtained from 10 streams (Água Boa, Cerro Verde, Cumprida, Ponte, Doradão, Guaçú, Guaporites, Nhu-Verá, Perobão and Santa Maria), one river (Iguatemi River) and nine ponds (Bacal, Balan, Ponte, Cinco, Macrófitas, Bugão, Sítio, Cascalho, and Oxbow). A total of 63 aquatic and semi-aquatic species of Heteroptera were recorded, of which 32 are new records.
water-bug; aquatic insects; Iguatemi River