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Jornal Brasileiro de Patologia e Medicina Laboratorial

Print version ISSN 1676-2444On-line version ISSN 1678-4774

Abstract

SOUZA, Gabriela Merheb de Azevedo; ROMANO, Nara Lúcia  and  WHITTLE, Martin R.. Efficient mispriming during apolipoprotein E genotyping. J. Bras. Patol. Med. Lab. [online]. 2005, vol.41, n.1, pp.25-28. ISSN 1676-2444.  http://dx.doi.org/10.1590/S1676-24442005000100007.

The determination of ApoE genotypes is a common clinical laboratory procedure. The most frequently used method involves a PCR amplification of the target region followed by a restriction enzyme digestion of the resultant amplicon to obtain a genotype-specific restriction fragment pattern. We describe an unexpected and surprising difficulty that was encountered during our routine laboratory casework.

Keywords : Polymerase chain reaction; Apolipoprotein E; ApoE genotyping.

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