Jornal Brasileiro de Patologia e Medicina Laboratorial
On-line version ISSN 1678-4774
TORRES, Júlio César Nogueira et al. Metallo-betalactamase producing Pseudomonas spp. strains isolated in the Hospital Geral de Fortaleza. J. Bras. Patol. Med. Lab. [online]. 2006, vol.42, n.5, pp.313-319. ISSN 1678-4774. http://dx.doi.org/10.1590/S1676-24442006000500003.
Pseudomonas sp. is a ubiquitous gram-negative bacilli, of free and frequent life in hospital environment. Metallo-betalactamases (MBLs) productive bacteria are largely resistant to betalactamics of wide spectrum, including cephalosporin and carbapenem. The objective of this work was to detect Pseudomonas spp. strains resistant to imipenem and ceftazidime, as well as to identify the MBLs producer ones. It was studied 311 isolated strains from several clinical samples at Fortaleza General Hospital (FGH), from June 2002 to June 2003. Identification and sensibility tests were done by the MicroScan®/WalkAway automation system. The multiresisting strains were confirmed by the diffusion method in disk. The triage to detect MBLs productive samples was accomplished by the double diffusion method, using disks containing sodium mercaptoacetat. Among these samples, 24 (7.71%) indicated production of MBLs and multiresistance standard in the midst of the studied strains. The antimicrobials to which the strains presented larger sensibility were piperacillin/tazobactam, with 255 (82%) of sensibility, followed by isolated piperacillin with 229 (73.63%); imipenem with 195 (62.70%); ticarcillin/clavulanic acid with 193 (62.05%); and ceftazidime with 138 (44.37%). The detection of these samples configures an emerging problem, with important implications in the antimicrobial therapeutic.
Keywords : Pseudomonas sp.; Metallo-betalactamases; Imipenem; Ceftazidime.