Jornal Brasileiro de Patologia e Medicina Laboratorial
Print version ISSN 1676-2444
COSTA, Carolina M. da; SANTOS, Rita C. C. dos and LIMA, Emerson S.. A simple automated procedure for thiol measurement in human serum samples . J. Bras. Patol. Med. Lab. [online]. 2006, vol.42, n.5, pp. 345-350. ISSN 1676-2444. http://dx.doi.org/10.1590/S1676-24442006000500006.
Thiol groups have been described as the main responsible for antioxidative effects of plasmatic proteins. Also, thiol serum levels have shown a positive correlation with total antioxidant capacity (TAC) in many studies. Measurement of TAC by substract oxidation-based methods have been widely used as a reference to measure antioxidant status; however, in many cases these methods are inexact or imprecise, usually when performed by manual procedures. In this paper we describe a simple automated procedure for the determination of total thiols in serum, which was based on Ellmans method. It was correlated with several markers of oxidative/antioxidative status, such as TAC and thiobarbituric acid reactive substance test (TBARs). Serum thiol levels were correlated positively with TAC (r = 0.298, p < 0.001) and negatively with TBARs levels (r = -0.330, p < 0.001). The novel automated procedure for thiol groups measurement can be a great tool in estimation of antioxidant status together with TAC assay. This procedure makes the determination of total thiol groups in large scale possible in clinical chemistry or research laboratories where this approach is necessary.
Keywords : Thiol groups; Total antioxidant capacity; TBARs; Oxidative stress; Automation.