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Jornal Brasileiro de Patologia e Medicina Laboratorial
Print version ISSN 1676-2444
On-line version ISSN 1678-4774
ROCHA, Rafael Malagoli et al. Construction of low cost tissue microarrays using alternative equipment for immunohistochemical study of breast tumors. J. Bras. Patol. Med. Lab. [online]. 2006, vol.42, n.6, pp.477-482. ISSN 1676-2444. http://dx.doi.org/10.1590/S1676-24442006000600012.
BACKGROUND: Tissue microarrays (TMA) are blocks containing numerous cylinders of paraffinized tissue organized in lines and columns allowing analysis of numerous samples in one slide. Commercially available equipment is imported and have high cost (US$ 11,000.00 to 24,000.00). AIM: we describe a low cost breast-tumor TMA and our experience in its use for immunohistochemistry (IHC). MATERIAL AND METHODS: A model that consists of a work station (Dremel) to which a liver biopsy needle of 2mm of diameter was connected. A receptor block was prepared perforating it until the desired number of rows (55) was reached. Then, the cylinders of tissue were obtained using the same equipment and included in the holes of the receptor block. Two samples were obtained from different tumor areas of each donor block. Cylinders of previously tested positive control tumors for each antibody and one marker (liver sample) that indicated the beginning of slide reading were also included. IHC was performed in sequential 4µm sections from the same array using antibodies against estrogen and progesterone receptors, Ki67, p53 and Her2. The first and the last slides were stained by hematoxylin and eosin to evaluate: number of tissue discs, tissue preservation, and adequacy of the tissue sample. RESULTS: The equipment total cost was US$ 180,00. The slides showed fine tissue preservation, adequate for morphologic evaluation, and sufficient to confirm diagnosis. The IHC quality was similar to the donor blocks. CONCLUSION: This equipment and technique represent an economical alternative when compared to commercial equipments.
Keywords : Tissue microarrays; Breast cancer; Immunohistochemistry; Prognostic markers.