Jornal Brasileiro de Patologia e Medicina Laboratorial
On-line version ISSN 1678-4774
ALMEIDA, Aline Silva et al. Reference values for blood-based biochemical parameters in BALB/C and C57BL/6 wild-type mice. J. Bras. Patol. Med. Lab. [online]. 2008, vol.44, n.6, pp.429-432. ISSN 1678-4774. http://dx.doi.org/10.1590/S1676-24442008000600006.
INTRODUCTION: Identifying individual biochemical parameters of laboratory animal species is important inasmuch as they may be used in the evaluation of functional changes in organs and in the establishment of reference values. OBJECTIVE: To establish biochemical reference values for blood tests in BALB/c and C57BL/6 wild-type mice from the Vivarium of the Department of Cellular Biology at the Federal University of "Triângulo Mineiro". MATERIALS AND METHODS: Thirty wild-type mice of the lineages BALB/c and C57BL/6 were used to evaluate the serum levels of glucose, triglycerides, cholesterol, total protein, albumin, amylase, uric acid, urea and alkaline phosphatase. The determinations were performed in a BIOPLUS-2000 analyzer. Results: Four out of the nine analytes (albumin, glucose, total proteins and cholesterol) showed significant statistical differences between the strains. Confidence interval (CI) values were standardized as reference values. In those analytes in which there was significant difference between strains (p < 0.05), confidence interval values of each lineage were adopted, whereas in those ones in which there were no differences, the minimum and maximum values of confidence interval from both lineages were applied. CONCLUSION: The results show the need for reference interval standardization of each Vivarium inasmuch as it reflects the conditions of the population in which the tests will be routinely performed.
Keywords : Laboratorial Analysis; Biochemical analysis; BALB/c; Wild-type C57BL/6; Reference values.