Abstract

BRITO JUNIOR, Lacy Cardoso de et al. Diagnosis of immunophenotyping of chronic lymphoproliferative syndromes by flow cytometry at HEMOPA blood center. J. Bras. Patol. Med. Lab. [online]. 2011, vol.47, n.6, pp. 607-610. ISSN 1676-2444.  http://dx.doi.org/10.1590/S1676-24442011000600006.

INTRODUCTION: Lymphoproliferative syndromes comprise a heterogeneous group of malignant neoplasias with different clinical behaviors, pathological factors and epidemiological characteristics, whose diagnosis may be based on lymphoid cell morphology observed in peripheral blood. OBJECTIVE: To test the diagnostic feasibility of immunophenotyping by flow cytometry for lymphoproliferative syndromes through the definition of minimal antibody panel. MATERIAL AND METHODS: During the period of July 2008 to July 2010, 47 patients from HEMOPA blood center participated in this study for differential diagnosis of lymphoproliferative syndromes subtypes by flow cytometry. RESULTS: The mean age was 68 years old. There was no statistical difference between genders, and the most frequent subtype of lymphoproliferative syndromes was chronic lymphoid leukemia/small B-cell lymphocytic lymphoma. CONCLUSION: Based on the antibody panel recommended in this investigation, the immunophenotyping method by flow cytometry associated with morphological characterization of peripheral blood samples is a reliable, rapid, feasible, and non-invasive procedure for the diagnosis of chronic lymphoproliferative syndromes.

Keywords : Flow cytometry; Immunophenotyping; Hematological neoplasias; Diagnosis; Lymphomas.