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Jornal Brasileiro de Patologia e Medicina Laboratorial

versão impressa ISSN 1676-2444


HERKENHOFF, Marcos Edgar et al. Prevalence of Chlamydia trachomatis in endocervical samples by PCR in São Paulo and Santa Catarina. J. Bras. Patol. Med. Lab. [online]. 2012, vol.48, n.5, pp.323-327. ISSN 1676-2444.

INTRODUCTION: No other sexually transmitted disease (STD) has been as frequent as Chlamydia trachomatis (CT) infection. Tubal damage caused by this agent has been frequently detected among women. This infection causes permanent infertility. Furthermore, surgical interventions have not demonstrated success in repairing tubal damage. The polymerase chain reaction (PCR) has proved to be more sensitive than culture to the identification of CT mainly in women with chlamydial cervicitis.PCR promotes the detection of specific nucleotide sequences in CT. OBJECTIVE: To analyze the prevalence of infections caused by CT in women in São Paulo and Santa Catarina states by use of endocervical samples. MATERIAL AND METHODS: In this study we used samples from laboratories in São Paulo and Santa Catarina states, which are associated with Genolab. CT examination result reports from 2010 obtained from Genolab database were analyzed. The phenol-chloroform protocol was used to obtain and isolate deoxyribonucleic acid (DNA) and the (PCR) method was used to amplify genetic material. RESULTS: We obtained a sample of 287 individuals, of which 56.45% were positive. Endocervical swab samples showed the highest positive results (75%). CONCLUSION: Endocervical samples constituted an accurate detection of CT. The high prevalence emphasizes the importance of molecular diagnosis, which is also corroborated by this study.

Palavras-chave : Chlamydia trachomatis; PCR; Endocervical.

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