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Jornal Brasileiro de Patologia e Medicina Laboratorial

versão impressa ISSN 1676-2444

Resumo

MENEZES, Maria das Graças Santos et al. HbA1c determination by HPLC: interference of hemoglobin variants HbS, HbC and HbF high concentration. J. Bras. Patol. Med. Lab. [online]. 2012, vol.48, n.5, pp.337-344. ISSN 1676-2444.  http://dx.doi.org/10.1590/S1676-24442012000500006.

INTRODUCTION: Diabetes mellitus (DM) is considered an important public health problem. It is highly prevalent and its incidence has progressively increased in recent years. OBJECTIVE: To verify possible variations of glycated hemoglobin (HbA1c) concentration in the presence of Hb S and Hb C and to evaluate the impact of HbA1c reduction on clinical evaluation and monitoring of diabetic patients. MATERIAL AND METHODS: This study comprised 150 diabetic individuals from Salvador city, Bahia, from both genders and average age of 56 years old. Fast blood glucose and HbA1c were determined by oxidase-peroxidase and high-performance liquid chromatography (HPLC) methods, respectively. RESULTS: There were variations in the concentration of HbA1c in the presence of hemoglobin variants such as AA (7.85%), AS (7.30%), and AC (7.15%). DISCUSSION AND CONCLUSION: The analytical method used to determine HbA1c needs to be chosen according to the general population characteristics and associated comorbidities, since the presence of hemoglobin S and C causes significant reductions in hemoglobin glycation, which may lead to clinical misinterpretation of patients' glycemic control.

Palavras-chave : Glycated hemoglobin; Variant hemoglobin; Methodological interference; HPLC.

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