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Jornal Brasileiro de Patologia e Medicina Laboratorial

versão impressa ISSN 1676-2444

Resumo

BORBA, Claudia Michele de Araujo; OLIVEIRA, Viviane Matoso de; AREND, Lavínia N. V. S.  e  PILONETTO, Marcelo. Validation of inhibition test by aminophenyl boronic acid to Klebsiella pneumoniae carbapenemases (KPC) screening. J. Bras. Patol. Med. Lab. [online]. 2012, vol.48, n.6, pp.427-433. ISSN 1676-2444.  http://dx.doi.org/10.1590/S1676-24442012000600007.

INTRODUCTION: The production of Klebsiella pneumoniae carbapenemases enzymes (KPC) has become an important and worrisome resistance mechanism. Furthermore, tests that combine high sensitivity and high specificity for the detection of these enzymes are scarce. OBJECTIVE: To validate the inhibition test by 3-aminophenyl boronic acid as a phenotypic screening method for KPC-producing strains by comparing the results with confirmatory polymerase chain reaction testing (PCR). METHODS: We evaluated the use of 3-aminophenyl boronic acid applied on disks with imipenem, meropenem and ertapenem antibiotics. 36 strains were positive and 12 were negative, all confirmed by PCR. Three different concentrations of boronic acid were also tested: 300, 400 and 600 µg. RESULTS: Among the positive strains, the results were more accurate with the addition of the compound to the ertapenem disk, presenting 100 % specificity and 50% sensitivity. CONCLUSION: Further studies are required, mainly regarding the standardization of the technique and materials, since the method seems to be promising as to the screening of KPC strains.

Palavras-chave : Aminophenyl boronic acid; Klebsiella pneumonia; Carbapenemases; KPC.

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