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International braz j urol

Print version ISSN 1677-5538On-line version ISSN 1677-6119

Abstract

KAJBAFZADEH, Abdol-Mohammad; SABETKISH, Shabnam  and  SABETKISH, Nastaran. The role of fetal-maternal microchimerism as a natural-born healer in integrity improvement of maternal damaged kidney. Int. braz j urol. [online]. 2018, vol.44, n.3, pp.608-616. ISSN 1677-5538.  https://doi.org/10.1590/s1677-5538.ibju.2017.0324.

Purpose:

To identify the fetal stem cell (FSC) response to maternal renal injury with emphasis on renal integrity improvement and Y chromosome detection in damaged maternal kidney.

Materials and Methods:

Eight non-green fluorescent protein (GFP) transgenic Sprague-Dawley rats were mated with GFP-positive transgenic male rats. Renal damage was induced on the right kidney at gestational day 11. The same procedure was performed in eight non-pregnant rats as control group. Three months after delivery, right ne- phrectomy was performed in order to evaluate the injured kidney. The fresh perfused kidneys were stained with anti-GFP antibody. Polymerase chain reaction (PCR) assay was also performed for the Y chromosome detection. Cell culture was performed to detect the GFP-positive cells. Technetium-99m-DMSA renal scan and single-photon emission computed tomography (SPECT) were performed after renal damage induction and 3 months later to evaluate the improvement of renal integrity.

Results:

The presence of FSCs was confirmed by immune histochemical staining as well as immunofluorescent imaging of the damaged part. Gradient PCR of female rat purified DNA demonstrated the presence of Y-chromosome in the damaged maternal kidney. Moreover, the culture of kidney cells showed GPF- positive cells by immuno- fluorescence microscopy. The acute renal scar was repaired and the integrity of dam- aged kidney reached to near normal levels in experimental group as shown in DMSA scan. However, no significant improvement was observed in control group.

Conclusion:

FSC seems to be the main mechanism in repairing of the maternal renal injury during pregnancy as indicated by Y chromosome and GFP-positive cells in the sub-cultured medium.

Keywords : Fetal Stem Cells; Y Chromosome; Technetium Tc 99m Dimercaptosuccinic Acid; Green Fluorescent Proteins.

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