SciELO - Scientific Electronic Library Online

vol.17 número2Insights into preventive measures for dental erosionInfluence of human and bovine substrate on the microleakage of two adhesive systems índice de autoresíndice de materiabúsqueda de artículos
Home Pagelista alfabética de revistas  

Servicios Personalizados




Links relacionados


Journal of Applied Oral Science

versión impresa ISSN 1678-7757versión On-line ISSN 1678-7765


ESTRELA, Carlos; SYDNEY, Gilson Blitzkow; FIGUEIREDO, José Antonio Poli  y  ESTRELA, Cyntia Rodrigues de Araújo. A model system to study antimicrobial strategies in endodontic biofilms. J. Appl. Oral Sci. [online]. 2009, vol.17, n.2, pp.87-91. ISSN 1678-7757.

The purpose of this work was to develop a model system to study antimicrobial strategies in endodontic biofilms. Enterococcus faecalis suspension was colonized in 10 human root canals. Five milliliters of Brain Heart Infusion (BHI) were mixed with 5 mL of the bacterial inoculums (E. faecalis) and inoculated with sufficient volume to fill the root canal during 60 days. This procedure was repeated every 72 h, always using 24-h pure culture prepared and adjusted to No. 1 MacFarland turbidity standard. Biofilm formation was analyzed by scanning electron microscopy (SEM). E. faecalis consistently adhered to collagen structure, colonized dentin surface, progressed towards the dentinal tubules and formed a biofilm. The proposed biofilm model seems to be viable for studies on antimicrobial strategies, and allows for a satisfactory colonization time of selected bacterial species with virulence and adherence properties.

Palabras clave : Biofilm; Endodontic biofilm; Enterococcus faecalis; Intracanal medicaments; Antimicrobial strategies.

        · texto en Inglés     · Inglés ( pdf epdf )


Creative Commons License Todo el contenido de esta revista, excepto dónde está identificado, está bajo una Licencia Creative Commons