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Journal of Applied Oral Science

Print version ISSN 1678-7757On-line version ISSN 1678-7765

Abstract

GRUNDLING, Grasiela Longhi et al. QMix® irrigant reduces lipopolysacharide (LPS) levels in an in vitro model. J. Appl. Oral Sci. [online]. 2015, vol.23, n.4, pp.431-435. ISSN 1678-7765.  https://doi.org/10.1590/1678-775720140488.

The presence of endotoxin inside the root canal has been associated with periapical inflammation, bone resorption and symptomatic conditions.

Objectives

To determine, in vitro, the effect of QMix® and other three root canal irrigants in reducing the endotoxin content in root canals.

Material and Methods

Root canals of single-rooted teeth were prepared. Samples were detoxified with Co-60 irradiation and inoculated with E. coli LPS (24 h, at 37°C). After that period, samples were divided into 4 groups, according to the irrigation solution tested: QMix®, 17% EDTA, 2% chlorhexidine solution (CHX), and 3% sodium hypochlorite (NaOCl). LPS quantification was determined by Limulus Amebocyte Lysate (LAL) assay. The initial counting of endotoxins for all samples, and the determination of LPS levels in non-contaminated teeth and in contaminated teeth exposed only to non-pyrogenic water, were used as controls.

Results

QMix® reduced LPS levels, with a median value of 1.11 endotoxins units (EU)/mL (p<0.001). NaOCl (25.50 EU/mL), chlorhexidine (44.10 EU/mL) and positive control group (26.80 EU/mL) samples had similar results. Higher levels were found with EDTA (176.00 EU/mL) when compared to positive control (p<0.001). There was no significant difference among EDTA, NaOCl and CHX groups. Negative control group (0.005 EU/mL) had statistically significant lower levels of endotoxins when compared to all test groups (p<0.001).

Conclusion

QMix® decreased LPS levels when compared to the other groups (p<0.001). 3% NaOCl, 2% CHX and 17% EDTA were not able to significantly reduce the root canal endotoxins load.

Keywords : Endotoxins; EDTA; Chlorhexidine; Sodium hypochlorite.

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