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Journal of Applied Oral Science

versão impressa ISSN 1678-7757versão On-line ISSN 1678-7765


ANDRUCIOLI, Marcela Cristina Damião et al. Successful and failed mini-implants: microbiological evaluation and quantification of bacterial endotoxin. J. Appl. Oral Sci. [online]. 2018, vol.26, e20170631.  Epub 10-Jul-2018. ISSN 1678-7757.


Using two groups of mini-implants (successful and failed) the objectives of this in vivo study were: to evaluate the microbial contamination by the checkerboard DNA-DNA hybridization technique and to quantify the bacterial endotoxin by the limulus amebocyte lysate assay.

Material and Methods

The 15 successful and 10 failed mini-implants (1.6 mm diameter × 7.0 or 9.0 mm long), placed in the maxilla and/or mandible, were obtained from 15 patients undergoing orthodontic treatment. Data were analyzed statistically by the Wilcoxon rank-sum test using the SAS software (a=0.05).


All 40 microbial species were detected in both groups of mini-implants, with different frequencies. No differences were observed between the groups with respect to microbial complexes (blue, purple, yellow, green, orange, red and other species) and endotoxin quantification (p>0.05).


Neither microbial contamination nor endotoxin quantification was determinant for the early loss of stability of the mini-implants.

Palavras-chave : Orthodontic anchorage procedures; Microbiology; Gram-negative bacteria.

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