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Journal of Venomous Animals and Toxins including Tropical Diseases

On-line version ISSN 1678-9199

Abstract

KARTHIKAYALU, S; RAMA, V  and  VENKATESAN, R. Purification of a 19-kDa pore-forming cytolysin from the sea anemone Heteractis magnifica. J. Venom. Anim. Toxins incl. Trop. Dis [online]. 2010, vol.16, n.3, pp.514-518. ISSN 1678-9199.  http://dx.doi.org/10.1590/S1678-91992010000300019.

Pore-forming cytolysins of 19 kDa from sea anemones present a remarkable cytolytic property. In the present work, a purified 19-kDa cytolysin was obtained from the sea anemone Heteractis magnifica. The purification steps involved ammonium sulfate precipitation and subsequently desalting by dialysis against 10 mM sodium phosphate buffer (pH 7.4), followed by anion exchange chromatography in DEAE-Sepharose® column (GE Healthcare, Sweden) and gel filtration chromatography using Sephadex® G-50 matrix (GE Healthcare, Sweden). The active fractions from the gel filtration chromatography were pooled and rechromatographed in the same column. The final active fraction showed a prominent protein band of molecular mass of 19 kDa when analyzed by SDS-PAGE.

Keywords : Heteractis magnifica; cytolysin; hemolysin; pore-forming toxin.

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