SciELO - Scientific Electronic Library Online

 
vol.18 número1Intradermal injection of Bothrops cotiara venom in mice in an experimental wound modelOrigin, transfer and distribution of cantharidin-related compounds in the blister beetle Hycleus scabiosae índice de autoresíndice de materiabúsqueda de artículos
Home Pagelista alfabética de revistas  

Journal of Venomous Animals and Toxins including Tropical Diseases

versión On-line ISSN 1678-9199

Resumen

RAHMAN, Mafruza S et al. Characterization of Clostridium perfringens isolated from mammals and birds from Guwahati city, India. J. Venom. Anim. Toxins incl. Trop. Dis [online]. 2012, vol.18, n.1, pp. 83-87. ISSN 1678-9199.  http://dx.doi.org/10.1590/S1678-91992012000100010.

Of the 102 samples collected from mammals and birds, both domestic and captive wild, 48 were found to be positive for Clostridium perfringens. Most of the mammal isolates (84.38%) appeared to have been collected from clinically affected animals, while 33.33% of the bird samples were from clinically affected and 21.43% from apparently healthy birds infected with C. perfringens. Isolates revealed high sensitivity to ciprofloxacin, enrofloxacin and norfloxacin. Among the isolated C. perfringens, 30 (62.50%) showed DNase production. Hemolytic activity was recorded in 14 (24.16%) of the isolates and 28 (58.33%) showed phospholipase C production. All the phospholipase C positive isolates revealed the presence of cpa gene encoding alpha (α) toxin. Of the 102 samples collected from mammals and birds, both domestic and captive wild, 48 were found to be positive for Clostridium perfringens. Most of the mammal isolates (84.38%) appeared to have been collected from clinically affected animals, while 33.33% of the bird samples were from clinically affected and 21.43% from apparently healthy birds infected with C. perfringens. Isolates revealed high sensitivity to ciprofloxacin, enrofloxacin and norfloxacin. Among the isolated C. perfringens, 30 (62.50%) showed DNase production. Hemolytic activity was recorded in 14 (24.16%) of the isolates and 28 (58.33%) showed phospholipase C production. All the phospholipase C positive isolates revealed the presence of cpa gene encoding α toxin.

Palabras llave : Clostridium perfringens; PCR; α toxin; cpa gene; polymerase chain reaction.

        · texto en Inglés     · pdf en Inglés