Journal of Venomous Animals and Toxins including Tropical Diseases
versión On-line ISSN 1678-9199
RODRIGUEZ, JP et al. Cross-neutralization of the coagulant activity of Crotalus durissus terrificus venom from the northeast of Argentina by bivalent bothropic antivenom. J. Venom. Anim. Toxins incl. Trop. Dis [online]. 2012, vol.18, n.1, pp.116-123. ISSN 1678-9199. http://dx.doi.org/10.1590/S1678-91992012000100015.
Cross-neutralization of Crotalus durissus terrificus venom coagulant activity was tested using bivalent horse antivenom against Bothrops alternatus and Bothrops diporus venoms. Our in vitro and in vivo experiments showed that bothropic antivenom neutralizes the thrombin-like activity of crotalic snake venom and this cross-reaction was demonstrated by immunoassays either with whole venom or a purified thrombin-like enzyme. These results suggest common antigenic properties and, consequently, similar molecular structure among venom thrombin-like enzymes. Besides, they provide information that could be further used in the development of new antivenom formulations.
Palabras clave : thrombin-like enzyme; snake venom; antivenoms; immunological cross-reaction.