Anticancer properties of phospholipase A₂ fromDaboia siamensis venom on human skin melanoma cells

Abstract

Background

Phospholipase A₂ (PLA₂) is a major component of theDaboia siamensis venom, which is able to hydrolyse the membrane of various cells. For this reason, the activity of PLA₂was investigated regarding its pharmaceutical properties. This study was conducted to explore the pharmacological properties of a PLA₂from Daboia siamensis (dssPLA₂) venom on human skin melanoma cell line (SK-MEL-28).

Methods

dssPLA₂ was isolated by ion exchange and gel filtration columns. Various concentrations of dssPLA₂were investigated for cytotoxic activity and inhibition of migration on SK-MEL-28 cells. Cell death analysis, mRNA expression levels of Notch I-III and BRAF V600E genes were also determined.

Results

dssPLA₂ exhibited cytotoxicity on SK-MEL-28 for 24 and 72 h as compared with untreated cells. However, it had no toxic effects on CCD-1064sk cells under the same conditions. dssPLA₂ (0.25 and 0.5 μg/mL) induced 17.16 and 30.60 % of apoptosis, while activated 6.53 and 7.05 % of necrotic cells. dssPLA₂ at 0.25, 0.5, 1 and 2 μg/mL could inhibit migration on SK-MEL-28 cells for 24 h by 31.06, 41.66, 50 and 68.75 %, respectively. The action of dssPLA₂ significantly reduced the levels of Notch I and BRAF V600E genes expression on SK-MEL-28 cells compared with untreated cells at 72 h.

Conclusions

This study indicates that dssPLA₂ had potential effects of apoptosis, necrosis, cytotoxicity and inhibition of migration on SK-MEL-28 cells. dssPLA₂ could possibly be a selective agent that targets cancer cells without affecting normal cells.

Phospholipase A₂; Daboia siamensis venom; Skin melanoma; Anticancer