Einstein (São Paulo)
Print version ISSN 1679-4508
FERREIRO-BARROS, Claudia Cristina; SUGAWARA, Eduardo Kinio and SANCHES, Livia Rentas. Determination of a method for extraction of coenzyme Q10 in human plasma: optimization of the use of surfactants and other variables. Einstein (São Paulo) [online]. 2012, vol.10, n.2, pp. 203-208. ISSN 1679-4508. http://dx.doi.org/10.1590/S1679-45082012000200014.
OBJECTIVE: To establish a routine for the extraction of the total levels of CoQ10 in human plasma through the Ultra High Performance Liquid Chromatography (UHPLC). METHODS: Two extraction protocols were tested: a) methanol: hexane and b) 1-propanol. The following parameters were analyzed: extraction temperature (19ºC and 4ºC), extraction tubes (glass and polypropylene), and surfactants (SDS, Triton X-100, Tween-20) at different concentrations, i.e., 1%, 3%, 5% and 10%. RESULTS: The results showed that the method of extraction of CoQ10 in a sample of human plasma at 4ºC, using solvents methanol: hexane (85:15, v/v) in the presence of surfactant Tween-20 at 3% and polypropylene tubes showed better efficiency and reproducibility when compared to the method with 1-propanol. CONCLUSION: By the analyses performed, it was possible to observe that the addition of the surfactant Tween-20 promoted an increase in the recovery of CoQ10 by the methanol:hexane extraction method. This method showed good reproducibility, with a low coefficient of variation and high sensitivity, since CoQ10 was detected in samples of plasma of a control individual using a UV-type detector. The use of UHPLC equipment allowed a total analysis with total run time of 3.5 minutes, enabling the rapid achievement of results, considered mandatory for laboratory routines.
Keywords : Coenzymes; Surfactants; Chromatography, liquid.