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Jornal Brasileiro de Pneumologia

Print version ISSN 1806-3713

Abstract

TAKHTFOOLADI, Mohammad Ashrafzadeh et al. Effect of tramadol on lung injury induced by skeletal muscle ischemia-reperfusion: an experimental study. J. bras. pneumol. [online]. 2013, vol.39, n.4, pp.434-439. ISSN 1806-3713.  https://doi.org/10.1590/S1806-37132013000400006.

OBJECTIVE:

To determine whether tramadol has a protective effect against lung injury induced by skeletal muscle ischemia-reperfusion.

METHODS:

Twenty Wistar male rats were allocated to one of two groups: ischemia-reperfusion (IR) and ischemia-reperfusion + tramadol (IR+T). The animals were anesthetized with intramuscular injections of ketamine and xylazine (50 mg/kg and 10 mg/kg, respectively). All of the animals underwent 2-h ischemia by occlusion of the femoral artery and 24-h reperfusion. Prior to the occlusion of the femoral artery, 250 IU heparin were administered via the jugular vein in order to prevent clotting. The rats in the IR+T group were treated with tramadol (20 mg/kg i.v.) immediately before reperfusion. After the reperfusion period, the animals were euthanized with pentobarbital (300 mg/kg i.p.), the lungs were carefully removed, and specimens were properly prepared for histopathological and biochemical studies.

RESULTS:

Myeloperoxidase activity and nitric oxide levels were significantly higher in the IR group than in the IR+T group (p = 0.001 for both). Histological abnormalities, such as intra-alveolar edema, intra-alveolar hemorrhage, and neutrophil infiltration, were significantly more common in the IR group than in the IR+T group.

CONCLUSIONS:

On the basis of our histological and biochemical findings, we conclude that tramadol prevents lung tissue injury after skeletal muscle ischemia-reperfusion.

Keywords : Tramadol; Muscle, skeletal; Ischemic attack, transient; Lung Injury.

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