SciELO - Scientific Electronic Library Online

vol.26 issue2Children's discomfort in assessments using different methods for approximal caries detectionInfluence of pulp condition on the accuracy of an electronic foramen locator in posterior teeth: an in vivo study author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand




Related links


Brazilian Oral Research

On-line version ISSN 1807-3107


LEITAO, Tarcísio Jorge; TENUTA, Livia Maria Andaló; ISHI, Guilherme  and  CURY, Jaime Aparecido. Calcium binding to S. mutans grown in the presence or absence of sucrose. Braz. oral res. [online]. 2012, vol.26, n.2, pp.100-105. ISSN 1807-3107.

Sucrose is the most cariogenic dietary carbohydrate because it is a substrate for insoluble extracellular polysaccharide (IEPS) production in dental biofilms, which can proportionally decrease bacterial density and, consequently, the number of biofilm calcium (Ca) binding sites. Ca bound to bacterial cell walls can be released into the biofilm fluid during a cariogenic challenge, reducing the driving force for mineral dissolution provoked by the pH drop. Thus, we investigated the effect of an IEPS-rich extracellular matrix on bacterial Ca binding after treatment with Ca solutions. Streptococcus mutans Ingbritt 1600 was cultivated in culture broths supplemented with 1.0% sucrose or 0.5% glucose + 0.5% fructose. The IEPS concentration in bacterial pellets was determined after alkaline extraction. Bacterial pellets were treated with 1 mM or 10 mM Ca++ solutions at 37ºC for 10 to 60 min. Ca binding to bacterial pellets, determined after acid extraction using the Arsenazo III reagent, was fast and concentration dependent. Although the IEPS concentration was approximately ten times higher in bacterial pellets cultivated in sucrose as compared to its monossaccharides, bound Ca concentration after Ca treatment was similar in both conditions. These results suggest that IEPS may not influence the amount of Ca bound to reservoirs of dental biofilms.

Keywords : Sucrose; Streptococcus mutans; Polysaccharides; Calcium; Dental Caries.

        · text in English     · English ( pdf epdf )


Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License