The effects of vardenafil and pentoxifylline administration in an animal model of ischemic colitis

Aziret, Mehmet; Irkorucu, Oktay; Reyhan, Enver; Erdem, Hasan; Das, Koray; Ozkara, Selvinaz; Surmelioglu, Ali; Sozen, Selim; Bali, Ilhan; Cetinkunar, Sulleyman; Deger, Kamuran Cumhur

doi:10.6061/clinics/2014(11)10

Acessibilidade / Reportar erro

Brasil

Español English

sumário « anterior atual seguinte »

Sumário

BASIC RESEARCH • Clinics 69 (11) • Nov 2014 • https://doi.org/10.6061/clinics/2014(11)10 copy

The effects of vardenafil and pentoxifylline administration in an animal model of ischemic colitis

Authorship SCIMAGO INSTITUTIONS RANKINGS

OBJECTIVES:

Vardenafil enhances dilatation of vascular smooth muscle and inhibits platelet aggregation. The purpose of this study was to evaluate the clinical effects of vardenafil and pentoxifylline administration in an experimental model of ischemic colitis.

METHODS:

Forty female Wistar albino rats weighing 250-300 g were randomized into five experimental groups (each with n = 8) as follows:1) a sham group subjected to a sham surgical procedure and administered only tap water; 2) a control group subjected to a standardized surgical procedure to induce ischemic colitis and administered only tap water; 3) and 4) treatment groups subjected to surgical induction of ischemic colitis followed by the postoperative administration of 5 mg/kg or 10 mg/kg vardenafil, respectively; and 5) a treatment group subjected to surgical induction of ischemic colitis followed by postoperative administration of pentoxifylline at 50 mg/kg/day per day as a single dose for a 3-day period. All animals were sacrificed at 72 h post-surgery and subjected to relaparotomy. We scored the macroscopically visible damage, measured the ischemic area and scored histopathology to determine the severity of ischemia. Tissue malondialdehyde levels were also quantified.

RESULTS:

The mean Gomella ischemic areas were 63.3 mm² in the control group; 3.4 and 9.6 mm² in the vardenafil 5 and vardenafil 10 groups, respectively; and 3.4 mm² in the pentoxifylline group (p= 0.0001). The mean malondialdehyde values were 63.7 nmol/g in the control group; 25.3 and 25.6 nmol/g in the vardenafil 5 and vardenafil 10 groups, respectively; and 22.8 nmol/g in the pentoxifylline group (p= 0.0001).

CONCLUSION:

Our findings indicate that vardenafil and pentoxifylline are effective treatment options in an animal model of ischemic colitis. The positive clinical effects produced by these drugs are likely due to their influence on the hemodynamics associated with vascular smooth muscle and platelet functions.

Ischemic Colitis; PDE-5 Inhibitors; Pentoxifylline; Vardenafil

Groups		Operation	Procedure	Euthanasia
Sham group	n:8	Only laparotomy	Oral 10 mg/kg 0.9% NaCl at the same time for 3 days	Cervical dislocation with 50 mg/kg ketamine after 72 hours.
Control group	n:8	Laparotomy and IC model	Oral 10 mg/kg 0.9% NaCl at the same time for 3 days
Vardenafil 5 mg group	n:8	Laparotomy and IC model	Oral 5 mg/kg vardenafil at the same time for 3 days
Vardenafil 10 mg group	n:8	Laparotomy and IC model	Oral 10 mg/kg vardenafil at the same time for 3 days
Pentoxifylline group	n:8	Laparotomy and IC model	I.M. 50 mg/kg PTX at the same time for 3 days

		Acidic liquid		Bowel dilatation		Serosal changes		Perforation		Adhesion
GROUPS		No	Yes	No	Yes	No	Yes	No	Yes	No	Yes
A = Sham group	n	8	0	8	0	8	0	8	0	8	0
	%	100	0	100	0	100	0	100	0	100	0
B = Control group	n	0	8	0	8	0	8	5	3	0	100
	%	0	100	0	100	0	100	62.5	37.5	0	100
C = Vardenafil 10 mg group	n	5	3	6	2	6	2	8	0	2	6
	%	62.5	37.5	75	25	75	25	100	0	25	75
D = Vardenafil 5 mg group	n	6	2	5	3	8	0	8	0	2	6
	%	75	25	62.5	37.5	100	0	100	0	25	75
E = Pentoxifylline group	n	8	0	6	2	8	0	8	0	3	5
	%	100	0	75	25	100	0	100	0	37.5	62.5
Statistics	p and binary comparison	A-B (0,001), B-C (0.026), B- (0.007), B- (0.0001)		A-B (0.001), B-C (0.007), B-D (0.026), B-E (0.007)		A-B (0.0001), B-C (0.007), B-D, (0001), B- (0.0001)		A-B (0.201), B-C (0.201), B-D (0.201), B-E (0.201)		A- (0.467), B-C (0.467), B-D (0.467), B-E (0.201)

		MDA (nmol/gr)	Wallace macroscopic damage scoring (MVD)						Chiu classification						Gomella ischemic areas (IAs) (mm²)
Groups	n	MDA (nmol/gr)	0	1	2	3	4	5	0	1	2	3	4	5	Gomella ischemic areas (IAs) (mm²)
A = Sham group	8	18.0	8	0	0	0	0	0	8	0	0	0	0	0	0.0
B = Control group	8	63.7	0	1	3	2	1	1	0	1	3	0	1	3	63.3
C = Vardenafil 10 mg	8	25.6	2	5	1	0	0	0	2	5	0	1	0	0	9.6
D = Vardenafil 5 mg	8	25.3	2	6	0	0	0	0	3	5	0	0	0	0	3.4
E = Pentoxifylline group	8	22.7	3	5	0	0	0	0	3	5	0	0	0	0	2.8
Binary group comparisons ( p -value)		A-B, B-C, B-D, B-E (p= 0.0001)	A-B (0.0001), A-C (0.003), A-D (0.003), A-E (0.009), B-C (0.003), B-D (0.001), B-E (0.001), C-D (0.679), C-E (0.424), D-E (0.602)						A-B (0.0001), A-C (0.003), A-D (0.009), A-E (0.009), B-C (0.006), B-D (0.001), B-E (0.001), C-D (0.424), C-E (0.424), D-E (0.333)						A-B, A-C, A-D, A-E, B-C, B-D, B-E p= 0.0001

Faculdade de Medicina / USP Rua Dr Ovídio Pires de Campos, 225 - 6 and., 05403-010 São Paulo SP - Brazil, Tel.: (55 11) 2661-6235 - São Paulo - SP - Brazil
E-mail: clinics@hc.fm.usp.br

Acompanhe os números deste periódico no seu leitor de RSS

[1] Aziret M contributed to project design and execution, experimental work, data collection, transcription and coordination. Irkorucu O contributed to project design, project execution and statistical evaluation of data. Reyhan E, Surmelioglu A and Sozen S contributed to project design, project execution and experimental work. Erdem H contributed to data collection and transcription. Das K, Bali I contributed to statistical analysis and data collection. Ozkara S performed the pathological examinations. Cetinkunar S contributed to data collection. Deger KC contributed to da analysis.