Evaluation of the effects of ischemic preconditioning
                    on the hematological parameters of rats subjected to intestinal ischemia and
                    reperfusion

Tahir, Muhammad; Arshid, Samina; Heimbecker, Ana Maria C.; Castro, Mariana S.; de Souza Montero, Edna Frasson; Fontes, Belchor; Fontes, Wagner

doi:10.6061/clinics/2015(01)11

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Sumário

Basic Researchs • Clinics 70 (1) • Jan 2015 • https://doi.org/10.6061/clinics/2015(01)11 copy

Evaluation of the effects of ischemic preconditioning on the hematological parameters of rats subjected to intestinal ischemia and reperfusion

Authorship SCIMAGO INSTITUTIONS RANKINGS

OBJECTIVES:

Intestinal ischemia/reperfusion often leads to acute lung injury and multiple organ failure. Ischemic preconditioning is protective in nature and reduces tissue injuries in animal and human models. Although hematimetric parameters are widely used as diagnostic tools, there is no report of the influence of intestinal ischemia/reperfusion and ischemic preconditioning on such parameters. We evaluated the hematological changes during ischemia/reperfusion and preconditioning in rats.

METHODS:

Forty healthy rats were divided into four groups: control, laparotomy, intestinal ischemia/reperfusion and ischemic preconditioning. The intestinal ischemia/reperfusion group received 45 min of superior mesenteric artery occlusion, while the ischemic preconditioning group received 10 min of short ischemia and reperfusion before 45 min of prolonged occlusion. A cell counter was used to analyze blood obtained from rats before and after the surgical procedures and the hematological results were compared among the groups.

RESULTS:

The results showed significant differences in hematimetric parameters among the groups. The parameters that showed significant differences included lymphocyte, white blood cells and granulocyte counts; hematocrit; mean corpuscular hemoglobin concentration; red cell deviation width; platelet count; mean platelet volume; plateletcrit and platelet distribution width.

CONCLUSION:

The most remarkable parameters were those related to leukocytes and platelets. Some of the data, including the lymphocyte and granulocytes counts, suggest that ischemic preconditioning attenuates the effect of intestinal ischemia/reperfusion on circulating blood cells. Our work contributes to a better understanding of the hematological responses after intestinal ischemia/reperfusion and IPC, and the present findings may also be used as predictive values.

Ischemic Reperfusion Injury; Ischemic Preconditioning; Systemic Inflammatory Response; Intestinal Ischemia; Hemocytometry; Superior Mesenteric Artery Occlusion

Parameters	Control group			Laparotomy group			Ischemia/Reperfusion group			Preconditioning group
Parameters	Mean ± std dev	Median	Range Min - Max	Mean ± std dev	Median	Range Min - Max	Mean ± std dev	Median	Range Min - Max	Mean ± std dev	Median	Range Min - Max
WBC (10⁹/L)	10.27±2.04	10.85	6.8-12.3	14.71±3.4	15.75	8.6-18.7	18.13±5.16	19.1	11.8-15.75	16.39±4.67	15.7	8.7-23.2
Lymphocytes (10⁹/L)	6.79±1.59	7.2	4.1-8.8	5.6±1.42	5.3	3.5-7.7	5.65±1.28	5.65	3.7-8.8	5.61±2.24	5.05	3.9-11.6
Monocytes (10⁹/L)	0.31±0.07	0.3	0.2-0.4	0.6±0.28	0.5	0.3-1.2	0.71±0.29	0.6	0.4-0.6	0.57±0.19	0.6	0.3-0.8
Granulocytes (10⁹/L)	3.17±0.59	3.1	2.3-3.9	8.51±2.11	9.15	4.7-10.7	13.36±6.31	12.85	5-9.15	10.21±3.7	9.85	4.5-17.6
Lymphocytes (%)	64.76±2.96	65.1	60-70.8	38.26±4.98	39.95	30.3-45.1	28.47±5.23	27.35	22-70.8	36.33±8.19	34.85	26.7-51.8
Monocytes (%)	3.25±0.36	3.1	2.9-3.9	3.68±0.6	3.6	2.9-4.7	3.36±0.28	3.35	3-3.9	3.81±0.43	3.85	3.2-4.7
Granulocytes (%)	32.1±2.86	31.65	26.2-37.1	57.63±4.61	56.75	51.1-65.9	67.87±5.74	69.5	57.5-57.63	59.89±8.25	61.45	44.5-69.3
RBC (10¹²/L)	6.528±0.55	6.58	5.49-7.52	7.38±0.72	7.65	6.1-8.3	7.66±0.52	7.665	6.88-7.65	7.32±1.38	7.63	4.62-9.58
Hb (g/L)	128±6.94	126.5	120-142	138.5±12.03	138.5	120-156	143.3±7.69	144	134-142	141±20.02	147.5	107-167
HCT (%)	40.83±1.86	40.45	38.8-44.4	44.63±3.63	44.95	38.7-49.8	47.07±2.61	46.85	43.3-44.95	45.14±6.31	47.65	34.2-54.2
MCV (fL)	61.39±2.46	61.1	57.2-65.1	60.41±1.95	59.95	58-63.7	61.57±2.34	61.2	58.2-65.1	60.8±2.39	60.75	56.6-64.7
MCH	19.16±0.75	19.15	17.6-20.3	18.6±0.67	18.6	17.8-19.7	18.7±0.8	18.8	17.5-20.3	18.98±0.73	19.05	17.4-20.1
MCHC (g/L)	312.8±4.61	312.5	307-320	308.7±5.89	308.5	301-320	304.1±5.36	302.5	297-320	312.7±7.12	313.5	303-325
RDW (%)	11.46±0.72	11.2	10.7-12.7	11.96±0.65	11.95	10.5-12.9	12.52±1.35	12.15	11-12.7	12.87±1.32	12.7	11.5-15.2
PLT (10⁹/L)	746.6±148.32	785.5	529-943	837.3±92.71	828	679-1024	867.2±154.25	800.5	734-943	653.1±248.34	665	341-1135
MPV (fL)	5.3±0.2	5.3	5-5.7	5.45±0.18	5.45	5.1-5.8	5.64±0.2	5.6	5.4-5.7	5.93±0.33	5.95	5.5-6.4
PDW	16.1±0.24	16.05	15.8-16.5	16.15±0.26	16.15	15.8-16.7	16.13±0.13	16.15	15.9-16.5	16.86±0.68	16.9	15.9-18.1
PCT (%)	0.3946±0.08	0.412	0.275-0.49	0.46±0.03	0.456	0.418-0.52	0.49±0.09	0.441	0.401-0.49	0.3763±0.12	0.3945	0.218-0.578

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[1] Tahir M, Arshid S and Montero EF were responsible for the hematimetric parameter analysis and surgical procedures. Heimbecker AM and Fontes B were responsible for the surgical procedures. Castro MS and Fontes W were responsible for the experimental design and statistical analysis. All of the authors contributed to the discussion of the results and to the writing of the article.