SciELO - Scientific Electronic Library Online

 
vol.73The influence of inspiratory muscle training combined with the Pilates method on lung function in elderly women: A randomized controlled trialUNIFESP Info Plastica – An informative application covering the most frequently performed plastic surgeries worldwide author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand

Journal

Article

Indicators

Related links

Share


Clinics

Print version ISSN 1807-5932On-line version ISSN 1980-5322

Abstract

VILALVA, Kelvin Henrique et al. Prophylactic application of laser light restores L-FABP expression in the livers of rats submitted to partial ischemia. Clinics [online]. 2018, vol.73, e113.  Epub July 02, 2018. ISSN 1807-5932.  http://dx.doi.org/10.6061/clinics/2018/e113.

OBJECTIVES:

The objective of the present study was to evaluate the protective effect of pre-conditioning treatment with laser light on hepatic injury in rats submitted to partial ischemia using mitochondrial function and liver fatty acid binding protein as markers.

METHODS:

Rats were divided into four groups (n=5): 1) Control, 2) Control + Laser, 3) Partial Ischemia and 4) Partial Ischemia + Laser. Ischemia was induced by clamping the hepatic pedicle of the left and middle lobes of the liver for 60 minutes. Laser light at 660 nm was applied to the liver immediately prior to the induction of ischemia at 22.5 J/cm2, with 30 seconds of illumination at five individual points. The animals were sacrificed after 30 minutes of reperfusion. Blood and liver tissues were collected for analysis of mitochondrial function, determination of malondialdehyde and analysis of fatty acid binding protein expression by Western blot.

RESULTS:

Mitochondrial function decreased in the Partial Ischemia group, especially during adenosine diphosphate-activated respiration (state 3), and the expression of fatty acid binding protein was also reduced. The application of laser light prevented bioenergetic changes and restored the expression of fatty acid binding protein.

CONCLUSION:

Prophylactic application of laser light to the livers of rats submitted to partial ischemia was found to have a protective effect in the liver, with normalization of both mitochondrial function and fatty acid binding protein tissue expression.

Keywords : Ischemia; Reperfusion Injury; Laser Therapy; L-FABP.

        · text in English     · English ( pdf )