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Arquivos do Instituto Biológico

On-line version ISSN 1808-1657


SALVARINI, Felipe Masiero et al. In vitro potency test for evaluation of Clostridium perfringens type D epsilon toxoid. Arq. Inst. Biol. [online]. 2013, vol.80, n.4, pp.450-452. ISSN 1808-1657.

The control of enterotoxemia caused by the epsilon toxin, produced by Clostridium perfringenstype D, is based on vaccination with epsilon toxoid. The potency test for this immunogen is conducted using seroneutralization in mice. Here, an in vitro test for detection of neutralizing antibodies with Madin-Darby Canine Kidney (MDCK) cells was standardized in order to study alternative methodologies for the potency test. Titers observed in the in vivo and in vitro seroneutralization tests had a correlation of 99.73%.

Keywords : seroneutralization; mouse; cell culture; bioethics; vaccine.

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