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Arquivos do Instituto Biológico

versão impressa ISSN 0020-3653versão On-line ISSN 1808-1657

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PINTO, Ciro Pedro Guidotti; RICKES, Letícia Neutzling; ZOTTI, Moisés João  e  GRUTZMACHER, Anderson Dionei. Compared activity of agonist molecules towards ecdysone receptor in insect cell-based screening system. Arq. Inst. Biol. [online]. 2019, vol.86, e0312019.  Epub 25-Nov-2019. ISSN 0020-3653.  https://doi.org/10.1590/1808-1657000312019.

The ecdysone receptor, naturally activated by steroidal hormones, is a key protein for molting and reproduction processes of insects. Artificial activation of such receptor by specific pesticides induces an anomalous process of ecdysis, causing death of insects by desiccation and starvation. In this paper, we established a protocol for screening agonistic molecules towards ecdysone receptor of insect cells line S2 (Diptera) and Sf9 (Lepidoptera), transfected with the reporter plasmid ere.b.act.luc. Therefore, we set dose-response curves with the ecdysteroid 20-hydroxyecdysone, the phytoecdysteroid ponasterone-A, and tebufenozide, a pesticide belonging to the class of diacylhydrazines. In both cell lines, the median effective concentration values on reporter gene induction (EC50) of ponasterone-A was the smallest, meaning the most active agonist molecule. In Sf9 cells, tebufenozide had as smaller EC50 than 20-hydroxyecdysone, indicating the high agonistic capability and lepidopteran specificity. The protocol established in this study can be useful for a quick screening and rational research of site-specific pesticides.

Palavras-chave : plasmid; tebufenozide; 20-hydroxyecdysone; ponasterone-A.

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