Print version ISSN 1808-1851
SOUZA, Fabiano Inácio de; BARROS FILHO, Tarcísio Eloy Pessoa and CRISTANTE, Alexandre Fogaça. Evaluation of the use of GM1 after experimental spinal cord injury in rats. Coluna/Columna [online]. 2011, vol.10, n.4, pp.305-308. ISSN 1808-1851. http://dx.doi.org/10.1590/S1808-18512011000400011.
OBJECTIVE: To evaluate the efficacy of GM1, administered intraperitoneally after experimental spinal cord injury in rats. METHODS: A total of 20 Wistar rats, young adults, males, with a mean age of 20 weeks, weighting about 350g were divided into two groups of 10 animals. The group 1 was given only saline intraperitoneally. Group 2 received GM1 30mg/kg daily, also intraperitoneally. The lesions were produced by following the international protocol MASCIS (Multicenter Animal Spinal Cord Injury Study), using the system NY Impactor. The animals were evaluated functionally by the BBB scale at days 14, 28 and 42 after injury. The evoked potential was performed on all animals 42 days after trauma. RESULTS: We used a two-factor variance model (ANOVA) and the Student t test. The functional assessments as well as those by evoked potential showed no statistically significant difference between the two groups. CONCLUSION: The use of intraperitoneal GM1 was not efficient after experimental spinal cord injury.
Keywords : Spinal cord injuries; G(M1) Ganglioside; Rats, Wistar.