Comparative study between biopsy and brushing sampling methods for detection of human papillomavirus in oral and oropharyngeal cavity lesions<b><sup>,</sup></b>

Marques, Marise da Penha Costa; Bussoloti Filho, Ivo; Rossi, Lia Mara; Andreoli, Maria Antonieta; Cruz, Natália Oliveira

doi:10.1016/j.bjorl.2015.08.007

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Brazilian Journal of Otorhinolaryngology

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ORIGINAL ARTICLES • Braz. j. otorhinolaryngol. 81 (6) • Nov-Dec 2015 • https://doi.org/10.1016/j.bjorl.2015.08.007 copy

Comparative study between biopsy and brushing sampling methods for detection of human papillomavirus in oral and oropharyngeal cavity lesions^☆ ☆ Please cite this article as: Marques MPC, Bussoloti Filho I, Rossi LM, Andreoli MA, Cruz NO. Comparative study between biopsy and brushing sampling methods for detection of human papillomavirus in oral and oropharyngeal cavity lesions. Braz J Otorhinolaryngol. 2015;81:598-603. ^,^{^☆☆} ☆☆ Institution: Faculdade de Ciências Médicas da Santa Casa de São Paulo (FCMSCSP), São Paulo, SP, Brazil.

Authorship SCIMAGO INSTITUTIONS RANKINGS

ABSTRACT

INTRODUCTION:

Many epidemiological studies have suggested that human papillomavirus (HPV), especially type 16, is involved in the genesis of squamous cell carcinoma of the oral cavity and oropharynx, especially in young, non-smoking patients; thus, its detection in lesions in this region is important.

OBJECTIVE:

To clarify the capacity of the brushing sampling method to detect the presence of HPV in oral or oropharyngeal lesions through polymerase chain reaction (PCR) testing, and to compare the results with those obtained by biopsy.

METHODS:

Prospective study of adult patients with oral or oropharyngeal lesions assessed by PCR, comparing biopsy specimens with samples obtained by the brushing method. The study was approved by the Research Ethics Committee of the institution.

RESULTS:

A total of 35 sample pairs were analyzed, but 45.7% of the brushing samples were inadequate (16/35) and, thus, only 19 pairs could be compared. There was agreement of results in 94.7% (18/19) of the pairs, with HPV identified in 16 of them. HPV DNA was detected in 8.6% (3/35) of biopsy and 5.7% (2/35) of brushing samples.

CONCLUSION:

There was no statistically significant difference between the two methods, but the brushing sampling method showed a higher number of inadequate samples, suggesting that it is an unreliable method for surveillance.

Keywords:
Polymerase chain reaction; Human papillomavirus DNA tests; Mouth; Oropharynx

Case	Characteristics of lesions	Brushing sample		Tissue sample
		ß-Globin	DNA HPV	ß-Globin	DNA HPV
1	Tongue SCC	Yes	No	Yes	No
2	Palatine tonsil SCC	Yes	No	Yes	No
3	Oral mucosa SCC	Yes	No	Yes	No
4	Palatine tonsil SCC	Yes	HPV-52	Yes	No
5	Ulcerated inflammatory process of palatine tonsil	Yes	No	Yes	No
6	Papillomatosis of oral cheek mucosa	Yes	No	Yes	No
7	Base of the tongue SCC	Yes	No	Yes	No
8	Ulcerated inflammatory process of the mouth floor	No	No	Yes	No
9	Hodgkin's lymphoma of base of the tongue	No	No	Yes	HPV-6
10	Palatine tonsil SCC	No	No	Yes	No
11	Soft palate papillomatosis	No	No	Yes	No
12	Soft palate papillomatosis	No	No	Yes	No
13	Mouth floor SCC	No	No	Yes	No
14	Lower-lip SCC	No	No	Yes	No
15	SCC of the base of the tongue	No	No	Yes	No
16	Ulcerated inflammatory process of the oral cheek mucosa	No	No	Yes	No
17	Tongue SCC	Yes	No	Yes	No
18	Soft palate papillomatosis	No	No	Yes	No
19	Palatine tonsil papillomatosis	No	No	Yes	No
20	SCC of the base of the tongue	No	No	Yes	No
21	Oropharyngeal squamous papilloma	No	No	Yes	HPV-11
22	Non-Hodgkin lymphoma of palatine tonsil	Yes	No	Yes	No
23	Soft palate SCC	Yes	No	Yes	No
24	Tongue SCC	Yes	No	Yes	No
25	Palatine tonsil SCC	No	No	Yes	No
26	Palatine tonsil SCC	Yes	No	Yes	No
27	Palatine tonsil SCC	Yes	HPV-16	Yes	HPV-16
28	SCC of the base of the tongue	Yes	No	Yes	No
29	Tongue squamous papilloma	No	No	Yes	No
30	Fibroepithelial polyp of the soft palate	Yes	No	Yes	No
31	Squamous papilloma of the anterior pillar	Yes	No	Yes	No
32	SCC of the uvula	Yes	No	Yes	No
33	Soft palate SCC	Yes	No	Yes	No
34	Tongue SCC	Yes	No	Yes	No
35	Soft palate SCC	No	No	Yes	No

Associação Brasileira de Otorrinolaringologia e Cirurgia Cérvico-Facial. Sede da Associação Brasileira de Otorrinolaringologia e Cirurgia Cérvico Facial, Av. Indianópolia, 1287, 04063-002 São Paulo/SP Brasil, Tel.: (0xx11) 5053-7500, Fax: (0xx11) 5053-7512 - São Paulo - SP - Brazil
E-mail: revista@aborlccf.org.br

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[1] * Corresponding author. E-mail:mariseorl@globo.com(M.P.C. Marques).