Revista Odonto Ciência
On-line version ISSN 1980-6523
CARVALHO, Suzana Papile Maciel; SALES-PERES, Arsenio; RIBEIRO-BICUDO, Lucilene Arilho and SILVA, Ricardo Henrique Alves da. Quality evaluation of DNA obtained from stored human saliva and its applicability to identification in Forensic Dentistry. Rev. odonto ciênc. (Online) [online]. 2010, vol.25, n.1, pp. 48-53. ISSN 1980-6523. http://dx.doi.org/10.1590/S1980-65232010000100010.
PURPOSE: This study evaluated the quality of DNA obtained from stored human saliva and its applicability to human identification. METHODS: The saliva samples of 20 subjects, collected in the form of saliva in natura and from mouth swabs and stored at -20ºC, were analyzed. After 7 days, the DNA was extracted from the 40 saliva samples and subjected to PCR and electrophoresis. After 180 days, the technique was repeated with the 20 swab samples. RESULTS: The first-stage results indicated that DNA was successfully extracted in 97.5% of reactions, 95% of saliva in natura and 100% of swab saliva samples, with no statistically significant difference between the forms of saliva. In the second phase, the result was positive for all 20 analyzed samples (100%). Subsequently, in order to analyze the quality of the DNA obtained from human saliva, the SIX3-2 gene was tested on the 20 mouth swab samples, and the PCR products were digested using the MbO1 restriction enzyme to evaluate polymorphisms in the ADRA-2 gene, with positive results for most samples. CONCLUSION: It was concluded that the quantity and quality of DNA from saliva and the techniques employed are adequate for forensic analysis of DNA.
Keywords : Human identification; Forensic Dentistry; Forensic Medicine; DNA; molecular biology; saliva.