SciELO - Scientific Electronic Library Online

 
vol.25 issue2Epidemiological analysis of nifedipine and phenytoin-induced gingival overgrowth in users of the Primary Health Care SystemRepair of amalgam restorations with conventional and bonded amalgam: an in vitro study author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand

Journal

Article

Indicators

Related links

Share


Revista Odonto Ciência

On-line version ISSN 1980-6523

Abstract

ABDALLA, Ligia Fernandes et al. Mycobacterium leprae in the periodontium, saliva and skin smears of leprosy patients. Rev. odonto ciênc. (Online) [online]. 2010, vol.25, n.2, pp.148-153. ISSN 1980-6523.  http://dx.doi.org/10.1590/S1980-65232010000200008.

PURPOSE: To verify the presence of M. leprae in the periodontium, saliva and skin slit smears of leprosy patients. To correlate bacteriological and molecular findings with clinical data and compare laboratory techniques. METHODS: A cross-sectional study was designed to use bacteriological (baciloscopy) and molecular (PCR) parameters to detect M. leprae in exudates of the gingival sulcus/periodontium pocket, saliva and skin slit smears from multiple clinical forms of leprosy patients without previous treatment. RESULTS: The study included 48 leprosy patients with 15 multibacillary (MB) cases and 33 paucibacillary (PB) cases. The diagnosis of MB was confirmed through bacteriological examination and PCR results from skin slit smears. A total of 16 (48.5%) PB patients were PCR positive only. Four PB patients with negative PCR skin smears were PCR positive for the periodontium and saliva, with 2 cases and 1 case, respectively. No periodontium or saliva samples had positive bacteriological results. CONCLUSION: There was no correlation between periodontal disease and the presence of M. leprae. Bacteriological examination did not prove to be an efficient technique for the analysis of saliva and periodontium samples. PCR analysis of skin smears was more efficient at diagnosing PB patients than bacteriological examination. PCR positive results for the detection of M. leprae in PB patients can be increased by collecting slit skin smears, periodontium and saliva samples.

Keywords : Mycobacterium leprae; skin smears; PCR; periodontium; saliva; bacteriological examination.

        · abstract in Portuguese     · text in English     · English ( pdf )

 

Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License