Tropical Plant Pathology
Print version ISSN 1982-5676
BOGO, Amauri; MAFFIOLETTI, Marta A.; SANHUEZA, Rosa M. V. and CASA, Ricardo T.. Morphological characterization of Cryptosporiopsis perennans isolates in different culture media. Trop. plant pathol. [online]. 2008, vol.33, n.3, pp. 248-251. ISSN 1982-5676. http://dx.doi.org/10.1590/S1982-56762008000300013.
The mycelial growth and conidial production of nine isolates of Cryptosporiopsis perennans were evaluated on the culture media of potato-dextrose-agar acidified (PDA-acid), malt extract agar (MEA) and V8-agar (V8) under 12 hours of photoperiod at 21°C. Five mm-diameter disks taken from colonies grown for 14 days on PDA medium under 12 hours of photoperiod at 21°C were transferred to Petri dishes with 20 mL of each medium. Colonial morphology, mycelial growth, sporulation and spore dimension were evaluated after 13 days of incubation. The effect of the culture media varied according to isolates. Mycelial growth and conidial production were observed in all tested media. The culture medium V8 was the best medium tested for mycelial growth and conidial production, followed by MEA for all C. perennans isolates. In all culture media the isolates presented a fusiform conidial shape.
Keywords : Malus domestica; apple; bull's-eye rot.