Tropical Plant Pathology
versão On-line ISSN 1983-2052
COSTAMILAN, Leila M. et al. First report of Diaporthe phaseolorum var. caulivora infecting soybean plants in Brazil. Trop. plant pathol. [online]. 2008, vol.33, n.5, pp. 381-385. ISSN 1983-2052. http://dx.doi.org/10.1590/S1982-56762008000500007.
In March 2006, diseased soybean plants (Glycine max) were found in the municipalities of Passo Fundo and Coxilha, state of Rio Grande do Sul, Brazil, showing withered, brown leaves and light to reddish-brown discoloration and necrosis (canker) of the lower half of the stem. Cultural characteristics in potato dextrose agar showed identical patterns to those described for Diaporthe phaseolorum varcaulivora (Dpc). The rDNA-ITS sequence data from the Brazilian strain CH 40/06 (GenBank accession number EU622854) were compared to homologous sequences from the NCBI GenBank database, and a high similarity with Dpc strains was verified. A phylogenetic reconstruction was performed to compare EU622854 with other related sequences and grouped with Dpc. This group was highly supported by the bootstrap test (99%). Morphology of the Brazilian specimen was also similar to that described in the literature for Dpc, but the ascospores were slightly narrower than the American original material of Dpc. In field surveys performed in the 2006/07 crop season, Dpc was also found in five additional municipalities in RS.
Palavras-chave : stem canker; Phomopsis phaseoli f.sp. caulivora; Glycine max.