Tropical Plant Pathology
Print version ISSN 1982-5676
WIN, Nang Kyu Kyu and JUNG, Hee-Young. 'Candidatus Phytoplasma cynodontis' associated with white leaf disease of golden beard grass (Chrysopogon acicalatus). Trop. plant pathol. [online]. 2012, vol.37, n.1, pp.76-79. ISSN 1982-5676. http://dx.doi.org/10.1590/S1982-56762012000100009.
A phytoplasma was detected in golden beard grass (Chrysopogon acicalatus) displaying white leaf symptoms near vegetative fields at the Tatkone region in Myanmar, 2011. Based on restriction fragment length polymorphism analysis of PCR-amplified 16S rRNA genes, including the 16S-23S spacer region and part of the 23S rRNA gene, the phytoplasma was identified as a member of the Bermuda grass white leaf phytoplasma (BGWL) group. The golden beard grass white leaf phytoplasma (GBGWL) 16S rRNA gene sequence exhibited over 98.7% similarity with all members of BGWL group phytoplasmas and 99.5% similarity with Thailand Bermuda grass white leaf phytoplasma (AF248961). In addition, the GBGWL phytoplasma was confirmed to be a member of 'Candidatus Phytoplasma cynodontis' by phylogenetic analyses.
Keywords : 'Candidatus Phytoplasma cynodontis'; golden beard grass; 16S rRNA gene.