Tropical Plant Pathology
Print version ISSN 1982-5676
LONDONO, Aurora; CAPOBIANCO, Heather; ZHANG, Shouan and POLSTON, Jane E.. First record of Tomato chlorotic spot virus in the USA. Trop. plant pathol. [online]. 2012, vol.37, n.5, pp. 333-338. Epub Sep 18, 2012. ISSN 1982-5676. http://dx.doi.org/10.1590/S1982-56762012005000001.
A tospovirus was identified in tomato plants from two counties in Florida by reverse transcription-PCR and sequencing of portions of the S, M and L genomic segments. The predicted amino-acid sequences of the N protein of PCR products from four plant samples were >96% identical to those of TCSV. Partial nucleic acid sequences of the L and M RNA were >97% identical to those reported for TCSV isolates. Extracts from field samples infected test plants and produced symptoms similar to those reported for TCSV. This is the first report of an isolate of TCSV in Florida and in the USA.
Keywords : Bunyaviridae; Solanum lycopersicum; South Florida; Tospovirus.