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vol.32 número1FUNGOS MICORRÍZICOS ARBUSCULARES E DOSES DE FÓSFORO NO CRESCIMENTO DO CAFEEIRO EM SOLO NÃO ESTERILIZADOQUALIDADE PÓS-COLHEITA DE FRUTOS DE MACAÚBA OZONIZADOS PARA A PRODUÇÃO BIODIESEL índice de autoresíndice de assuntospesquisa de artigos
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Revista Caatinga

versão impressa ISSN 0100-316Xversão On-line ISSN 1983-2125

Resumo

ALMEIDA NETO, Jorge Xavier de; REGO, Mailson Monteiro do; REGO, Elizanilda Ramalho do  e  SILVA, Ana Paula Gomes da. GENETIC DIVERSITY AMONG AND WITHIN BRAVE BEAN (Capparis flexuosa L.) POPULATIONS ASSESSED USING RAPD MARKERS. Rev. Caatinga [online]. 2019, vol.32, n.1, pp.81-91.  Epub 09-Maio-2019. ISSN 0100-316X.  http://dx.doi.org/10.1590/1983-21252019v32n109rc.

Brave bean (Capparis flexuosa L.) is a Caatinga species that is used as forage, mainly during the dry season when some plant species lose their leaves. The aim of this study was to assess genetic diversity within and among brave bean populations using Random Amplified Polymorphic DNA (RAPD) markers. Brave bean leaves were collected from 30 accessions in the following municipalities of Paraíba state, Brazil: Barra de Santa Rosa (BSR), Cuité (C), São João do Cariri (SJC), Damião (D), Baraúna (B), and Picuí (P). DNA extraction followed the standard methodology of CTAB with modifications. RAPD analyses were carried out using 18 primers, and polymorphism of the amplified DNA fragments was visualized using agarose gel electrophoresis. Data were used to calculate Jaccard Similarity Coefficient values, which were then used to group samples with the Unweighted Pair Group Method with Arithmetic Mean. Cophenetic Correlation Coefficient, Stress, and Distortion Coefficient values were also calculated from these analyses. Band polymorphism was generated with 14 primers, but the sampled populations showed low numbers of polymorphic loci (27 in BSR, 18 in C, 7 in SJC, 9 in D, and 0 in B and P). The highest polymorphic information content was found in samples from the BSR (9 groups), C (22 groups), SJC (7 groups), and D (6 groups) municipalities. In the interpopulation analysis, 34 groups were formed, the matrices of which showed high cophenetic correlations (0.95 to 0.98), but low stress (12.9 to 17.45%) and distortion (3.05%). Therefore, results showed that there was genetic variability both among and within brave bean populations.

Palavras-chave : Biotechnology; Caatinga; Molecular marker; Species conservation.

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