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Revista Brasileira de Parasitologia Veterinária

On-line version ISSN 1984-2961

Abstract

DA SILVA, Aleksandro Schafer et al. Trypanocidal activity of human plasma on Trypanosoma evansi in mice. Rev. Bras. Parasitol. Vet. [online]. 2012, vol.21, n.1, pp. 55-59. ISSN 1984-2961.  http://dx.doi.org/10.1590/S1984-29612012000100011.

This study aimed to test an alternative protocol with human plasma to control Trypanosoma evansi infection in mice. Plasma from an apparently 27-year-old healthy male, blood type A+, was used in the study. A concentration of 100 mg.dL-1 apolipoprotein L1 (APOL1) was detected in the plasma. Forty mice were divided into four groups with 10 animals each. Group A comprised uninfected animals. Mice from groups B, C and D were inoculated with a T. evansi isolate. Group B was used as a positive control. At three days post-infection (DPI), the mice were administered intraperitoneally with human plasma. A single dose of 0.2 mL plasma was given to those in group C. The mice from group D were administered five doses of 0.2 mL plasma with a 24 hours interval between the doses. Group B showed high increasing parasitemia that led to their death within 5 DPI. Both treatments eliminated parasites from the blood and increased the longevity of animals. An efficacy of 50 (group C) and 80% (group D) of human plasma trypanocidal activity was found using PCR. This therapeutic success was likely achieved in the group D due to their higher levels of APOL1 compared with group C.

Keywords : Trypanosoma evansi; treatment; human plasma; APOL1.

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