<i>Ehrlichia canis</i> and <i>Rickettsia</i> spp. in dogs from urban areas in Paraiba state, northeastern Brazil

Rotondano, Tereza Emmanuelle de Farias; Krawczak, Felipe da Silva; Barbosa, Werona de Oliveira; Moraes-Filho, Jonas; Bastos, Fernanda Nieri; Labruna, Marcelo Bahia; Azevedo, Sérgio Santos de; Melo, Marcia Almeida de; Almeida, Alzira Maria Paiva de

doi:10.1590/S1984-29612017030

Acessibilidade / Reportar erro

Brasil

Revista Brasileira de Parasitologia Veterinária

Español English

Brasil

Español English

sumário « anterior atual seguinte »

Sumário

Original Article • Rev. Bras. Parasitol. Vet. 26 (2) • Apr-Jun 2017 • https://doi.org/10.1590/S1984-29612017030 copy

Ehrlichia canis and Rickettsia spp. in dogs from urban areas in Paraiba state, northeastern Brazil

Ehrlichia canis e Rickettsia spp. em cães de áreas urbanas da Paraíba, nordeste do Brasil

Authorship SCIMAGO INSTITUTIONS RANKINGS

Abstract

The aims of our study was to identify Ehrlichia canis and antibodies against Rickettsia spp. belonging to the spotted fever group (SFG) in dogs sampled from Paraiba state, northeastern Brazil. Blood and serum samples collected by convenience from dogs in urban areas of five municipalities were analyzed by real-time PCR for the detection of E. canis DNA and by immunofluorescence assay test (IFAT) for the identification of antibodies against Rickettsia rickettsii, R. felis, R. parkeri, R. amblyommii and R. rhipicephali antigens. E. canis DNA was detected in 8.9% (64/719) of the blood samples, whereas 5.63% (43/763) of the serum samples were positive for at least one of the Rickettsia antigens tested by IFAT. This study showed for the first time the occurrence of E. canis and suggested the circulation of SFG Rickettsia in dogs in the study region of Paraiba state, northeastern Brazil.

Keywords:
Ehrlichia spp.; Rickettsia spp.; IFAT; PCR; Brazil

Municipalities	Number positive/number of analyzed samples, (% positivity)
	Assay
	Ehrlichia canis (real-time PCR)	Rickettsia spp. (IFA)
Uiraúna	14/100 (1.94%)	2/100 (2.00%)
Cajazeiras	7/74 (0.97%)	2/97 (2.06%)
Sousa	4/68 (0.55%)	5/89 (5.61%)
Areia	15/101 (2.08%)	4/101 (3.96%)
Campina Grande	24/376 (3.33%)	30/376 (7.97%)
Total	64/719 (8.9%)	43/763 (5.63%)

Municipalities	Nº Tested dogs	Nº Positive dogs (%)	Number of dogs reactive to each species of Rickettsia (% positivity)					PAIHR
Municipalities	Nº Tested dogs	Nº Positive dogs (%)	*R. rickettsii*	*R. parkeri*	*R. rhipicephali*	*R. amblyommii*	*R. felis*	PAIHR
Uiraúna	100	2 (2)	0 (0)	1 (1)	0 (0)	1 (1)	0 (0)	0
Cajazeiras	97	2 (2.06)	1 (1.03)	0 (0)	2 (2.06)	1 (1.03)	0 (0)	0
Sousa	89	5 (5.62)	2 (2.24)	4 (4.49)	1 (1.12)	2 (2.24)	0 (0)	0
Areia	101	4 (3.96)	1 (0.99)	2 (1.98)	2 (1.98)	3 (2.97)	1(0.99)	2:1 R. amblyommii, 1 R. felis
Campina Grande	376	30 (7.98)	9 (2.39)	13(3.45)	7 (1.86)	16 (4.25)	2(0.23)	1 R. rickettsii
Total	763	43 (5.63)	13 (1.7)	20 (2.62)	12 (1.57)	23 (3.01)	3(0.39)

Colégio Brasileiro de Parasitologia Veterinária FCAV/UNESP - Departamento de Patologia Veterinária, Via de acesso Prof. Paulo Donato Castellane s/n, Zona Rural, , 14884-900 Jaboticabal - SP, Brasil, Fone: (16) 3209-7100 RAMAL 7934 - Jaboticabal - SP - Brazil
E-mail: cbpv_rbpv.fcav@unesp.br

Acompanhe os números deste periódico no seu leitor de RSS

[1] *Corresponding author: Tereza Emmanuelle de Farias Rotondano. Departamento de Ciências Veterinárias, Centro de Ciências Agrárias, Universidade Federal da Paraíba – UFPB, Rodovia BR 079, Km 12, CEP 58397-000, Areia, PB, Brasil. e-mail: terezarotondano@hotmail.com