Crop Breeding and Applied Biotechnology
On-line version ISSN 1984-7033
ROSADO, Tatiana Barbosa et al. Detection of QTL associated with rust resistance using IBD-based methodologies in exogamic Eucalyptus spp. populations. Crop Breed. Appl. Biotechnol. (Online) [online]. 2010, vol.10, n.4, pp. 321-328. ISSN 1984-7033. http://dx.doi.org/10.1590/S1984-70332010000400006.
In Brazil the rust caused by Puccinia psidii Winter stands out as the most important disease of eucalyptus. The use of resistant genotypes is the main control method, which makes the detection of markers linked to rust resistance essential to the selection of resistant genotypes. In this study, an F1 progeny of 131 plants from interspecific crossings of Eucalyptus was used to identify markers linked to resistance genes for this pathogen. An integrated map was constructed for linkage group three based on microsatellite markers. For QTL mapping two methodologies based on alleles identical-by-descent (IBD) were used: single marker analysis of Haseman and Elston and the interval mapping procedure of Fulker and Cardon. Both methods showed significant association for the Embra 125 marker.The QTL that explained 42 % of the phenotypic variation was mapped to 0.02 cM of this marker by the Fulker and Cardon. Marker Embra 125 has potential use in assisted selection, thus increasing the efficiency of the selection of resistant genotypes.
Keywords : Eucalyptus grandis; Eucalyptus urophylla; Puccinia psidi; QTL mapping; molecular marker.