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Crop Breeding and Applied Biotechnology

versão On-line ISSN 1984-7033

Resumo

SALDANHA, Cleber Witt  e  MARTINS-CORDER, Maisa Pimentel. In vitro germination and embryogenic competence acquisition of Euterpe edulis Martius immature zygotic embryos. Crop Breed. Appl. Biotechnol. [online]. 2012, vol.12, n.3, pp.171-178. ISSN 1984-7033.  http://dx.doi.org/10.1590/S1984-70332012000300002.

This study evaluated different aspects of in vitro germination and embryogenic competence of immature zygotic embryos of E. edulis. The embryos germinated on full or half-strength MS (MS or MS/2) medium combined with sucrose (20, 30 and 40 g L-1). The effect of calcium chloride concentrations (0, 2, 4, 8, and 12 mM) on the induction of somatic embryogenesis was tested. The embryos were germinated on MS or MS/2. Germination of zygotic embryos and the number of roots per plantlet were not affected by the culture medium and sucrose concentration. Plantlet height and fresh weight were influenced by both; the difference was greatest in MS medium with 40 g L-1 sucrose. The induction of somatic embryogenesis was not influenced by doses of calcium chloride, whereas the number of somatic embryos formed was affected. The germination capacity of somatic embryos of heart-of-palm tree was not influenced by the media tested.

Palavras-chave : Arecaceae; zygotic embryo; micropropagation; morphogenesis; calcium chloride.

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