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Crop Breeding and Applied Biotechnology

Print version ISSN 1518-7853On-line version ISSN 1984-7033

Abstract

KNEIB, Raquel Bartz; KNEIB, Roberta Bartz; PEREIRA, Arione da Silva  and  CASTRO, Caroline Marques. Allele dosage of PVY resistance genes in potato clones using molecular markers. Crop Breed. Appl. Biotechnol. [online]. 2017, vol.17, n.4, pp.306-312. ISSN 1518-7853.  https://doi.org/10.1590/1984-70332017v17n4a47.

Potato virus Y (PVY) is considered one of the most important pathogens in potato crop. The objective of this study was to estimate the allele dosage of Ry genes and select clones with multiple copies of resistance alleles to be used as parents in potato breeding programs. Seven clonal families were genotyped, including five parents with resistance to PVY. For the allele Ryadg, based on the marker RYSC3, four parents are simplex, and one is duplex. For the allele Rysto, based on the marker M45, two parents are simplex and one is duplex. For two parents, the observed frequencies of the allele Rysto did not statistically adjust to any of the proposed genotypic constitutions. Based on the results obtained, both markers must be used in marker assisted selection for resistance to PVY, in order to decrease the error of discarding clones with only one of the alleles.

Keywords : Solanum tuberosum L.; genetic improvement; Potato virus Y; RYSC3; M45.

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