QTL mapping to anthracnose leaf blight resistance in tropical maize

Romanek, Cristiane; Matiello, Rodrigo Rodrigues; Coelho, Caroline de Jesus; Schafascheck, Lilian; Silva, Danilo Fernando Guimarães; Gardingo, José Raulindo

doi:10.1590/1984-70332017v17n4a58

Acessibilidade / Reportar erro

Brasil

Crop Breeding and Applied Biotechnology

Español English

Brasil

Español English

sumário « anterior atual seguinte »

Sumário

ARTICLE • Crop Breed. Appl. Biotechnol. 17 (4) • Dec 2017 • https://doi.org/10.1590/1984-70332017v17n4a58 copy

QTL mapping to anthracnose leaf blight resistance in tropical maize

Authorship SCIMAGO INSTITUTIONS RANKINGS

Abstract

The objectives were to map genomic regions associated with quantitative trait loci (QTL) to anthracnose leaf blight (ALB) in tropical maize, and to determine the effects of the loci on resistance to the pathogenic fungus Colletotrichum graminicola (Ces.). QTL analysis to ALB was carried out in a population of F_2:3 progenies resulting from the cross between the contrasting lines L_R 04-2 and L_S 95-1. Seventeen QTL were located by mapping analysis by composite interval on eight chromosomes. Four QTL mapped on chromosomes 9 (1 QTL) and 10 (3 QTL) were the most stable, detected in at least two experiments, and represented the most part of the phenotypic variation (27.7 to 54.3%) of resistance. The majority of QTL mapped were associated to specific severity evaluations/development stage of maize growth, and may explain the quantitative inheritance of resistance of tropical maize to ALB.

Key words:
Disease resistance; Colletotrichum graminicola; composite interval mapping; Zea mays; AUDPC

QTL Number	Chr	Markers Interval/ Position (cM)			1^st Experiment				2^nd Experiment				3^rd Experiment
QTL Number	Chr	Markers Interval/ Position (cM)			E1	E2	E3	AUDPC	E1	E2	E3	AUDPC	E1	E2	E3	AUDPC
1	1	E32M48_308	E42M50_174	LOD						31.3				15.3
		177.9	189.4	R² (%)						90.6				89.7
				a						- 0.55				- 0.55
2	2	E35M56_680	E35M56_112	LOD	31.6
		0.0	14.1	R² (%)	94.1
				a	0.52
3	3	E42M51_162	E42M50_76	LOD						18.4
		0.0	7.6	R² (%)						90.6
				a						0.55
4	3	E32M48_167	E32M59_104	LOD						11.5
		51.0	61.4	R² (%)						75.0
				a						0.49
5	4	E35M60_87	E32M60_185	LOD	10.8		10.7
		0.0	10.4	R² (%)	70.3		71.7
				a	0.41		0.35
6	4	E32M52_73	E44M51_84	LOD			21.6
		15.3	34.8	R² (%)			90.6
				a			0.07
7	4	Umc1511	E32M53_434	LOD						32.3
		88.1	119.3	R² (%)						90.6
				a						0.55
8	4	E32M53_434	E44M51_135	LOD			24.8
		119.3	137.7	R² (%)			90.4
				a			0.06
9	5	E32M48_532	E32M50_139	LOD						7.3
		242.0	244.3	R² (%)						61.8
				a						- 0.50
10	8	E35M60_80	E32M50_100	LOD	15.0					17.2				8.2
		0.0	23.9	R² (%)	72.6					74.8				67.5
				a	- 0.45					- 0.50				- 0.44
11	8	E32M60_94	E32M50_248	LOD	43.1		8.0	5.6		29.8				8.8	4.5	8.8
		57.3	74.1	R² (%)	94.0		72.5	52.2		90.6				69.1	23.4	50.9
				a	0.52		0.46	15.79		0.55				0.46	1.60	22.64
12	8	E35M60_86	Phi015	LOD									36.5
		85.5	107.5	R² (%)									87.5
				a									- 0.49
13	9	E32M48_562	E32M48_97	LOD	2.8			2.9		6.2		3.9		2.8	6.1	7.7
		126.4	157.7	R² (%)	18.0			27.7		32.8		33.8		33.0	34.0	33.8
				a	- 0.90			- 19.90		- 0.92		- 20.68		- 0.91	- 1.35	- 26.79
14	9	E32M51_314	E35M56_174	LOD						5.8						6.24
		179.1	201.1	R² (%)						32.8						37.8
				a						0.92						28.0
15	10	E32M49_698	E32M59_207	LOD	39.7		3.9	5.5				5.6		4.9	7.0	11.1
		28.3	58.7	R² (%)	94.1		30.8	39.4				41.5		34.9	41.4	50.8
				a	0.52		0.85	19.0				19.7		0.85	1.45	23.18
16	10	E32M50_118	E44M56_81	LOD			2.6	3.9				3.7		3.6		9.8
		85.4	109.1	R² (%)			29.6	35.0				33.0		33.1		52.1
				a			- 0.86	- 20.2				- 20.61		- 0.89		- 24.01
17	10	E32M59_76	Umc1084	LOD	45.1		18.5	7.3		33.4		7.7		18.36	6.0	9.6
	161.6		191.7	R² (%)	94.1		90.0	48.3		90.6		49.1		89.7	35.3	54.3
				a	0.52		0.55	17.6		0.55		18.16		0.55	1.42	20.35
	Number of intervals				7	0	7	5	0	10	0	4	1	7	4	6

Crop Breeding and Applied Biotechnology Universidade Federal de Viçosa, Departamento de Fitotecnia, 36570-000 Viçosa - Minas Gerais/Brasil, Tel.: (55 31)3899-2611, Fax: (55 31)3899-2611 - Viçosa - MG - Brazil
E-mail: cbab@ufv.br

Acompanhe os números deste periódico no seu leitor de RSS

[1] *E-mail: rrmatiel@uepg.br