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Crop Breeding and Applied Biotechnology

versão impressa ISSN 1518-7853versão On-line ISSN 1984-7033

Resumo

MONDIN, Mateus; SILVA, Paulo Artur Konzen Xavier de Mello e; LATADO, Rodrigo Rocha  e  MOURAO FILHO, Francisco de Assis Alves. In vitro induction and regeneration of tetraploids and mixoploids of two cassava cultivars. Crop Breed. Appl. Biotechnol. [online]. 2018, vol.18, n.2, pp.176-183. ISSN 1518-7853.  http://dx.doi.org/10.1590/1984-70332018v18n2a25.

We report the in vitro induction of polyploids from two cassava cultivars by colchicine treatment. Shoot nodal segments, collected from in vitro cultivated plants of Porquinho and Vassourinha cultivars, were used as explants. Explants were treated in liquid media without and with colchicine (0.05, 0.10 or 0.15%), at 90 rpm, in the dark, for 48 or 96 h. Normal-like and putative-polyploid phenotypes were recovered from in vitro culture, for both cultivars. All plants from the putative-polyploid group were confirmed as tetraploids by flow cytometry and root tip chromosome counting, whereas no association was found between ploidy level and phenotype in normal-like plants. Mixoploids were also recovered from both cultivars. Vassourinha was more responsive to colchicine treatment than Porquinho. Tetraploid plants have fewer, but bigger, stomata guard cells than those in diploids. Colchicine at 0.10% for 96 hours induced a high number of tetraploids in both cultivars.

Palavras-chave : Chimera; chromosome doubling; colchicine; Manihot esculenta; polyploidy.

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